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dc.contributor.authorBarney, Lauren E.
dc.contributor.authorHall, Christopher L.
dc.contributor.authorSchwartz, Alyssa D.
dc.contributor.authorParks, Akia N.
dc.contributor.authorSparages, Christopher
dc.contributor.authorGalarza, Sualyneth
dc.contributor.authorPlatt, Manu O.
dc.contributor.authorMercurio, Arthur M.
dc.contributor.authorPeyton, Shelly R.
dc.date2022-08-11T08:09:56.000
dc.date.accessioned2022-08-23T16:49:13Z
dc.date.available2022-08-23T16:49:13Z
dc.date.issued2020-03-11
dc.date.submitted2020-04-16
dc.identifier.citation<p>Barney LE, Hall CL, Schwartz AD, Parks AN, Sparages C, Galarza S, Platt MO, Mercurio AM, Peyton SR. Tumor cell-organized fibronectin maintenance of a dormant breast cancer population. Sci Adv. 2020 Mar 11;6(11):eaaz4157. doi: 10.1126/sciadv.aaz4157. PMID: 32195352; PMCID: PMC7065904. <a href="https://doi.org/10.1126/sciadv.aaz4157">Link to article on publisher's site</a></p>
dc.identifier.issn2375-2548 (Linking)
dc.identifier.doi10.1126/sciadv.aaz4157
dc.identifier.pmid32195352
dc.identifier.urihttp://hdl.handle.net/20.500.14038/41417
dc.description.abstractTumors can undergo long periods of dormancy, with cancer cells entering a largely quiescent, nonproliferative state before reactivation and outgrowth. To understand the role of the extracellular matrix (ECM) in regulating tumor dormancy, we created an in vitro cell culture system with carefully controlled ECM substrates to observe entrance into and exit from dormancy with live imaging. We saw that cell populations capable of surviving entrance into long-term dormancy were heterogeneous, containing quiescent, cell cycle-arrested, and actively proliferating cells. Cell populations capable of entering dormancy formed an organized, fibrillar fibronectin matrix via alphavbeta3 and alpha5beta1 integrin adhesion, ROCK-generated tension, and TGFbeta2 stimulation, and cancer cell outgrowth after dormancy required MMP-2-mediated fibronectin degradation. We propose this approach as a useful, in vitro method to study factors important in regulating dormancy, and we used it here to elucidate a role for fibronectin deposition and MMP activation.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=32195352&dopt=Abstract">Link to Article in PubMed</a></p>
dc.rightsCopyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subjecttumors
dc.subjectextracellular matrix (ECM)
dc.subjecttumor dormancy
dc.subjectbreast cancer
dc.subjectCancer Biology
dc.subjectCell Biology
dc.subjectNeoplasms
dc.titleTumor cell-organized fibronectin maintenance of a dormant breast cancer population
dc.typeJournal Article
dc.source.journaltitleScience advances
dc.source.volume6
dc.source.issue11
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=5218&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/4199
dc.identifier.contextkey17380830
refterms.dateFOA2022-08-23T16:49:13Z
html.description.abstract<p>Tumors can undergo long periods of dormancy, with cancer cells entering a largely quiescent, nonproliferative state before reactivation and outgrowth. To understand the role of the extracellular matrix (ECM) in regulating tumor dormancy, we created an in vitro cell culture system with carefully controlled ECM substrates to observe entrance into and exit from dormancy with live imaging. We saw that cell populations capable of surviving entrance into long-term dormancy were heterogeneous, containing quiescent, cell cycle-arrested, and actively proliferating cells. Cell populations capable of entering dormancy formed an organized, fibrillar fibronectin matrix via alphavbeta3 and alpha5beta1 integrin adhesion, ROCK-generated tension, and TGFbeta2 stimulation, and cancer cell outgrowth after dormancy required MMP-2-mediated fibronectin degradation. We propose this approach as a useful, in vitro method to study factors important in regulating dormancy, and we used it here to elucidate a role for fibronectin deposition and MMP activation.</p>
dc.identifier.submissionpathoapubs/4199
dc.contributor.departmentDepartment of Molecular, Cell and Cancer Biology
dc.source.pageseaaz4157


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Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
Except where otherwise noted, this item's license is described as Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.