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dc.contributor.authorLu, Aiping
dc.contributor.authorWatkins, Maren
dc.contributor.authorLi, Qing
dc.contributor.authorRobinson, Samuel D.
dc.contributor.authorConcepcion, Gisela P.
dc.contributor.authorYandell, Mark
dc.contributor.authorWeng, Zhiping
dc.contributor.authorOlivera, Baldomero M.
dc.contributor.authorSafavi-Hemami, Helena
dc.contributor.authorFedosov, Alexander E.
dc.date2022-08-11T08:09:56.000
dc.date.accessioned2022-08-23T16:49:17Z
dc.date.available2022-08-23T16:49:17Z
dc.date.issued2020-04-25
dc.date.submitted2020-05-08
dc.identifier.citation<p>Lu A, Watkins M, Li Q, Robinson SD, Concepcion GP, Yandell M, Weng Z, Olivera BM, Safavi-Hemami H, Fedosov AE. Transcriptomic profiling reveals extraordinary diversity of venom peptides in unexplored predatory gastropods of the genus Clavus. Genome Biol Evol. 2020 Apr 25:evaa083. doi: 10.1093/gbe/evaa083. Epub ahead of print. PMID: 32333764. <a href="https://doi.org/10.1093/gbe/evaa083">Link to article on publisher's site</a></p>
dc.identifier.issn1759-6653 (Linking)
dc.identifier.doi10.1093/gbe/evaa083
dc.identifier.pmid32333764
dc.identifier.urihttp://hdl.handle.net/20.500.14038/41432
dc.description.abstractPredatory gastropods of the superfamily Conoidea number over 12,000 living species. The evolutionary success of this lineage can be explained by the ability of conoideans to produce complex venoms for hunting, defense and competitive interactions. Whereas venoms of cone snails (family Conidae) have become increasingly well studied, the venoms of most other conoidean lineages remain largely uncharacterized. In the present study we present the venom gland transcriptomes of two species of the genus Clavus that belong to the family Drilliidae. Venom gland transcriptomes of two specimens of Clavus canalicularis, and two specimens of Cv. davidgilmouri were analyzed, leading to the identification of a total of 1,176 putative venom peptide toxins ("drillipeptides"). Based on the combined evidence of secretion signal sequence identity, entire precursor similarity search (BLAST), and the orthology inference, putative Clavus toxins were assigned to 158 different gene families. The majority of identified transcripts comprise signal, pro-, mature peptide, and post- regions, with a typically short ( < 50 amino acids) and cysteine-rich mature peptide region. Thus drillipeptides are structurally similar to conotoxins. However, convincing homology with known groups of Conus toxins was only detected for very few toxin families. Among these are Clavus counterparts of Conus venom insulins (drillinsulins), porins (drilliporins), highly diversified lectins (drillilectins). The short size of most drillipeptpides and structural similarity to conotoxins was unexpected, given that most related conoidean gastropod families (Terebridae and Turridae) possess longer mature peptide regions. Our findings indicate that, similar to conotoxins, drillipeptides may represent a valuable resource for future pharmacological exploration.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=32333764&dopt=Abstract">Link to Article in PubMed</a></p>
dc.rights© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectClavus
dc.subjectConoidea
dc.subjectdrillipeptides
dc.subjecttranscriptome
dc.subjectvenom
dc.subjectvenom gland
dc.subjectAmino Acids, Peptides, and Proteins
dc.subjectBiochemical Phenomena, Metabolism, and Nutrition
dc.subjectBiochemistry
dc.subjectBioinformatics
dc.subjectComputational Biology
dc.subjectEcology and Evolutionary Biology
dc.subjectGenomics
dc.subjectIntegrative Biology
dc.subjectMolecular Biology
dc.titleTranscriptomic profiling reveals extraordinary diversity of venom peptides in unexplored predatory gastropods of the genus Clavus
dc.typeAccepted Manuscript
dc.source.journaltitleGenome biology and evolution
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=5231&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/4212
dc.identifier.contextkey17677189
refterms.dateFOA2022-08-23T16:49:17Z
html.description.abstract<p>Predatory gastropods of the superfamily Conoidea number over 12,000 living species. The evolutionary success of this lineage can be explained by the ability of conoideans to produce complex venoms for hunting, defense and competitive interactions. Whereas venoms of cone snails (family Conidae) have become increasingly well studied, the venoms of most other conoidean lineages remain largely uncharacterized. In the present study we present the venom gland transcriptomes of two species of the genus Clavus that belong to the family Drilliidae. Venom gland transcriptomes of two specimens of Clavus canalicularis, and two specimens of Cv. davidgilmouri were analyzed, leading to the identification of a total of 1,176 putative venom peptide toxins ("drillipeptides"). Based on the combined evidence of secretion signal sequence identity, entire precursor similarity search (BLAST), and the orthology inference, putative Clavus toxins were assigned to 158 different gene families. The majority of identified transcripts comprise signal, pro-, mature peptide, and post- regions, with a typically short ( < 50 amino acids) and cysteine-rich mature peptide region. Thus drillipeptides are structurally similar to conotoxins. However, convincing homology with known groups of Conus toxins was only detected for very few toxin families. Among these are Clavus counterparts of Conus venom insulins (drillinsulins), porins (drilliporins), highly diversified lectins (drillilectins). The short size of most drillipeptpides and structural similarity to conotoxins was unexpected, given that most related conoidean gastropod families (Terebridae and Turridae) possess longer mature peptide regions. Our findings indicate that, similar to conotoxins, drillipeptides may represent a valuable resource for future pharmacological exploration.</p>
dc.identifier.submissionpathoapubs/4212
dc.contributor.departmentProgram in Bioinformatics and Integrative Biology


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© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
Except where otherwise noted, this item's license is described as © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.