rad21 Is Involved in Corneal Stroma Development by Regulating Neural Crest Migration
Authors
Zhang, Bi NingLiu, Yu
Yang, Qichen
Leung, Pui Ying
Wang, Chengdong
Wong, Thomas Chi Bun
Tham, Clement C.
Chan, Sun On
Pang, Chi Pui
Chen, Li Jia
Dekker, Job
Zhao, Hui
Chu, Wai Kit
UMass Chan Affiliations
Department of Biochemistry and Molecular PharmacologyProgram in Systems Biology
Document Type
Journal ArticlePublication Date
2020-10-21Keywords
Xenopus laeviscorneal stroma
neural crest migration
rad21
Biochemistry
Cell Biology
Developmental Biology
Embryonic Structures
Eye Diseases
Molecular Biology
Nucleic Acids, Nucleotides, and Nucleosides
Structural Biology
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Show full item recordAbstract
Previously, we identified RAD21(R450C) from a peripheral sclerocornea pedigree. Injection of this rad21 variant mRNA into Xenopus laevis embryos disrupted the organization of corneal stroma fibrils. To understand the mechanisms of RAD21-mediated corneal stroma defects, gene expression and chromosome conformation analysis were performed using cells from family members affected by peripheral sclerocornea. Both gene expression and chromosome conformation of cell adhesion genes were affected in cells carrying the heterozygous rad21 variant. Since cell migration is essential in early embryonic development and sclerocornea is a congenital disease, we studied neural crest migration during cornea development in X. laevis embryos. In X. laevis embryos injected with rad21 mutant mRNA, neural crest migration was disrupted, and the number of neural crest-derived periocular mesenchymes decreased significantly in the corneal stroma region. Our data indicate that the RAD21(R450C) variant contributes to peripheral sclerocornea by modifying chromosome conformation and gene expression, therefore disturbing neural crest cell migration, which suggests RAD21 plays a key role in corneal stroma development.Source
Zhang BN, Liu Y, Yang Q, Leung PY, Wang C, Wong TCB, Tham CC, Chan SO, Pang CP, Chen LJ, Dekker J, Zhao H, Chu WK. rad21 Is Involved in Corneal Stroma Development by Regulating Neural Crest Migration. Int J Mol Sci. 2020 Oct 21;21(20):7807. doi: 10.3390/ijms21207807. PMID: 33096935; PMCID: PMC7594026. Link to article on publisher's site
DOI
10.3390/ijms21207807Permanent Link to this Item
http://hdl.handle.net/20.500.14038/41625PubMed ID
33096935Related Resources
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© 2020 by the authors. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly citedDistribution License
http://creativecommons.org/licenses/by/4.0/ae974a485f413a2113503eed53cd6c53
10.3390/ijms21207807
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Except where otherwise noted, this item's license is described as © 2020 by the authors. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited