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dc.contributor.authorVasquez-Rifo, Alejandro
dc.contributor.authorRicci, Emiliano P.
dc.contributor.authorAmbros, Victor R.
dc.date2022-08-11T08:09:57.000
dc.date.accessioned2022-08-23T16:50:25Z
dc.date.available2022-08-23T16:50:25Z
dc.date.issued2020-12-01
dc.date.submitted2020-12-30
dc.identifier.citation<p>Vasquez-Rifo A, Ricci EP, Ambros V. Pseudomonas aeruginosa cleaves the decoding center of Caenorhabditis elegans ribosomes. PLoS Biol. 2020 Dec 1;18(12):e3000969. doi: 10.1371/journal.pbio.3000969. PMID: 33259473; PMCID: PMC7707567. <a href="https://doi.org/10.1371/journal.pbio.3000969" target="_blank" title="article on publisher's site">View article on publisher's site</a></p>
dc.identifier.issn1545-7885
dc.identifier.doi10.1371/journal.pbio.3000969
dc.identifier.pmid33259473
dc.identifier.urihttp://hdl.handle.net/20.500.14038/41655
dc.description.abstractPathogens such as Pseudomonas aeruginosa advantageously modify animal host physiology, for example, by inhibiting host protein synthesis. Translational inhibition of insects and mammalian hosts by P. aeruginosa utilizes the well-known exotoxin A effector. However, for the infection of Caenorhabditis elegans by P. aeruginosa, the precise pathways and mechanism(s) of translational inhibition are not well understood. We found that upon exposure to P. aeruginosa PA14, C. elegans undergoes a rapid loss of intact ribosomes accompanied by the accumulation of ribosomes cleaved at helix 69 (H69) of the 26S ribosomal RNA (rRNA), a key part of ribosome decoding center. H69 cleavage is elicited by certain virulent P. aeruginosa isolates in a quorum sensing (QS)-dependent manner and independently of exotoxin A-mediated translational repression. H69 cleavage is antagonized by the 3 major host defense pathways defined by the pmk-1, fshr-1, and zip-2 genes. The level of H69 cleavage increases with the bacterial exposure time, and it is predominantly localized in the worm's intestinal tissue. Genetic and genomic analysis suggests that H69 cleavage leads to the activation of the worm's zip-2-mediated defense response pathway, consistent with translational inhibition. Taken together, our observations suggest that P. aeruginosa deploys a virulence mechanism to induce ribosome degradation and H69 cleavage of host ribosomes. In this manner, P. aeruginosa would impair host translation and block antibacterial responses.
dc.language.isoen_US
dc.publisherPublic Library of Science
dc.relation<p><a href="https://pubmed.ncbi.nlm.nih.gov/33259473/" target="_blank" title="view article in PubMed">View article in PubMed</a></p>
dc.rightsCopyright: © 2020 Vasquez-Rifo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectPseudomonas aeruginosa
dc.subjectRibosomal RNA
dc.subjectCaenorhabditis elegans
dc.subjectRibosomes
dc.subjectVirulence factors
dc.subjectBiochemistry, Biophysics, and Structural Biology
dc.subjectBiology
dc.subjectDevelopmental Biology
dc.subjectPathogenic Microbiology
dc.titlePseudomonas aeruginosa cleaves the decoding center of Caenorhabditis elegans ribosomes
dc.typeJournal Article
dc.source.journaltitlePLoS biology
dc.source.volume18
dc.source.issue12
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=5476&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/4446
dc.identifier.contextkey20879127
refterms.dateFOA2022-08-23T16:50:25Z
html.description.abstract<p>Pathogens such as Pseudomonas aeruginosa advantageously modify animal host physiology, for example, by inhibiting host protein synthesis. Translational inhibition of insects and mammalian hosts by P. aeruginosa utilizes the well-known exotoxin A effector. However, for the infection of Caenorhabditis elegans by P. aeruginosa, the precise pathways and mechanism(s) of translational inhibition are not well understood. We found that upon exposure to P. aeruginosa PA14, C. elegans undergoes a rapid loss of intact ribosomes accompanied by the accumulation of ribosomes cleaved at helix 69 (H69) of the 26S ribosomal RNA (rRNA), a key part of ribosome decoding center. H69 cleavage is elicited by certain virulent P. aeruginosa isolates in a quorum sensing (QS)-dependent manner and independently of exotoxin A-mediated translational repression. H69 cleavage is antagonized by the 3 major host defense pathways defined by the pmk-1, fshr-1, and zip-2 genes. The level of H69 cleavage increases with the bacterial exposure time, and it is predominantly localized in the worm's intestinal tissue. Genetic and genomic analysis suggests that H69 cleavage leads to the activation of the worm's zip-2-mediated defense response pathway, consistent with translational inhibition. Taken together, our observations suggest that P. aeruginosa deploys a virulence mechanism to induce ribosome degradation and H69 cleavage of host ribosomes. In this manner, P. aeruginosa would impair host translation and block antibacterial responses.</p>
dc.identifier.submissionpathoapubs/4446
dc.contributor.departmentProgram in Molecular Medicine
dc.source.pagese3000969


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Copyright: © 2020 Vasquez-Rifo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Except where otherwise noted, this item's license is described as Copyright: © 2020 Vasquez-Rifo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.