Show simple item record

dc.contributor.authorSharma, Kriti
dc.contributor.authorPalatinszky, Marton
dc.contributor.authorNikolov, Georgi
dc.contributor.authorBerry, David
dc.contributor.authorShank, Elizabeth A
dc.date2022-08-11T08:09:57.000
dc.date.accessioned2022-08-23T16:50:27Z
dc.date.available2022-08-23T16:50:27Z
dc.date.issued2020-11-03
dc.date.submitted2021-01-04
dc.identifier.citation<p>Sharma K, Palatinszky M, Nikolov G, Berry D, Shank EA. Transparent soil microcosms for live-cell imaging and non-destructive stable isotope probing of soil microorganisms. Elife. 2020 Nov 3;9:e56275. doi: 10.7554/eLife.56275. PMID: 33140722; PMCID: PMC7609051. <a href="https://doi.org/10.7554/eLife.56275">Link to article on publisher's site</a></p>
dc.identifier.issn2050-084X (Linking)
dc.identifier.doi10.7554/eLife.56275
dc.identifier.pmid33140722
dc.identifier.urihttp://hdl.handle.net/20.500.14038/41664
dc.description.abstractMicroscale processes are critically important to soil ecology and biogeochemistry yet are difficult to study due to soil's opacity and complexity. To advance the study of soil processes, we constructed transparent soil microcosms that enable the visualization of microbes via fluorescence microscopy and the non-destructive measurement of microbial activity and carbon uptake in situ via Raman microspectroscopy. We assessed the polymer Nafion and the crystal cryolite as optically transparent soil substrates. We demonstrated that both substrates enable the growth, maintenance, and visualization of microbial cells in three dimensions over time, and are compatible with stable isotope probing using Raman. We applied this system to ascertain that after a dry-down/rewetting cycle, bacteria on and near dead fungal hyphae were more metabolically active than those far from hyphae. These data underscore the impact fungi have facilitating bacterial survival in fluctuating conditions and how these microcosms can yield insights into microscale microbial activities.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=33140722&dopt=Abstract">Link to Article in PubMed</a></p>
dc.rightsCopyright Sharma et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectB. subtilis
dc.subjectecology
dc.subjectfluorescence microscopy
dc.subjectinfectious disease
dc.subjectmicrobial ecology
dc.subjectmicrobiology
dc.subjectmucor fragilis
dc.subjectraman microspectroscopy
dc.subjectsoil-like microcosms
dc.subjectwet/dry cycles
dc.subjectEnvironmental Microbiology and Microbial Ecology
dc.subjectSystems Biology
dc.subjectTerrestrial and Aquatic Ecology
dc.titleTransparent soil microcosms for live-cell imaging and non-destructive stable isotope probing of soil microorganisms
dc.typeJournal Article
dc.source.journaltitleeLife
dc.source.volume9
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=5485&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/4455
dc.identifier.contextkey20925980
refterms.dateFOA2022-08-23T16:50:27Z
html.description.abstract<p>Microscale processes are critically important to soil ecology and biogeochemistry yet are difficult to study due to soil's opacity and complexity. To advance the study of soil processes, we constructed transparent soil microcosms that enable the visualization of microbes via fluorescence microscopy and the non-destructive measurement of microbial activity and carbon uptake in situ via Raman microspectroscopy. We assessed the polymer Nafion and the crystal cryolite as optically transparent soil substrates. We demonstrated that both substrates enable the growth, maintenance, and visualization of microbial cells in three dimensions over time, and are compatible with stable isotope probing using Raman. We applied this system to ascertain that after a dry-down/rewetting cycle, bacteria on and near dead fungal hyphae were more metabolically active than those far from hyphae. These data underscore the impact fungi have facilitating bacterial survival in fluctuating conditions and how these microcosms can yield insights into microscale microbial activities.</p>
dc.identifier.submissionpathoapubs/4455
dc.contributor.departmentDepartment of Microbiology and Physiological Systems
dc.contributor.departmentProgram in Systems Biology
dc.source.pagese56275


Files in this item

Thumbnail
Name:
elife_56275_v1.pdf
Size:
7.088Mb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record

Copyright Sharma et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Except where otherwise noted, this item's license is described as Copyright Sharma et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.