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dc.contributor.authorBrossas, Caroline
dc.contributor.authorValton, Anne-Laure
dc.contributor.authorVenev, Sergey V.
dc.contributor.authorChilaka, Sabarinadh
dc.contributor.authorCounillon, Antonin
dc.contributor.authorLaurent, Marc
dc.contributor.authorGoncalves, Coralie
dc.contributor.authorDuriez, Benedicte
dc.contributor.authorPicard, Franck
dc.contributor.authorDekker, Job
dc.contributor.authorPrioleau, Marie-Noelle
dc.date2022-08-11T08:09:57.000
dc.date.accessioned2022-08-23T16:50:28Z
dc.date.available2022-08-23T16:50:28Z
dc.date.issued2020-11-02
dc.date.submitted2021-01-04
dc.identifier.citation<p>Brossas C, Valton AL, Venev SV, Chilaka S, Counillon A, Laurent M, Goncalves C, Duriez B, Picard F, Dekker J, Prioleau MN. Clustering of strong replicators associated with active promoters is sufficient to establish an early-replicating domain. EMBO J. 2020 Nov 2;39(21):e99520. doi: 10.15252/embj.201899520. Epub 2020 Sep 16. PMID: 32935369; PMCID: PMC7604622. <a href="https://doi.org/10.15252/embj.201899520">Link to article on publisher's site</a></p>
dc.identifier.issn0261-4189 (Linking)
dc.identifier.doi10.15252/embj.201899520
dc.identifier.pmid32935369
dc.identifier.urihttp://hdl.handle.net/20.500.14038/41665
dc.description.abstractVertebrate genomes replicate according to a precise temporal program strongly correlated with their organization into A/B compartments. Until now, the molecular mechanisms underlying the establishment of early-replicating domains remain largely unknown. We defined two minimal cis-element modules containing a strong replication origin and chromatin modifier binding sites capable of shifting a targeted mid-late-replicating region for earlier replication. The two origins overlap with a constitutive or a silent tissue-specific promoter. When inserted side-by-side, these modules advance replication timing over a 250 kb region through the cooperation with one endogenous origin located 30 kb away. Moreover, when inserted at two chromosomal sites separated by 30 kb, these two modules come into close physical proximity and form an early-replicating domain establishing more contacts with active A compartments. The synergy depends on the presence of the active promoter/origin. Our results show that clustering of strong origins located at active promoters can establish early-replicating domains.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=32935369&dopt=Abstract">Link to Article in PubMed</a></p>
dc.rightsCopyright 2020 The Authors. Published under the terms of the CC BY NC ND 4.0 license.
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectchromatin accessibility
dc.subjectnuclear organization
dc.subjectpromoter
dc.subjectreplication origin
dc.subjectreplication timing
dc.subjectBiochemistry, Biophysics, and Structural Biology
dc.subjectGenetics and Genomics
dc.subjectSystems Biology
dc.titleClustering of strong replicators associated with active promoters is sufficient to establish an early-replicating domain
dc.typeJournal Article
dc.source.journaltitleThe EMBO journal
dc.source.volume39
dc.source.issue21
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=5486&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/4456
dc.identifier.contextkey20925981
refterms.dateFOA2022-08-23T16:50:28Z
html.description.abstract<p>Vertebrate genomes replicate according to a precise temporal program strongly correlated with their organization into A/B compartments. Until now, the molecular mechanisms underlying the establishment of early-replicating domains remain largely unknown. We defined two minimal cis-element modules containing a strong replication origin and chromatin modifier binding sites capable of shifting a targeted mid-late-replicating region for earlier replication. The two origins overlap with a constitutive or a silent tissue-specific promoter. When inserted side-by-side, these modules advance replication timing over a 250 kb region through the cooperation with one endogenous origin located 30 kb away. Moreover, when inserted at two chromosomal sites separated by 30 kb, these two modules come into close physical proximity and form an early-replicating domain establishing more contacts with active A compartments. The synergy depends on the presence of the active promoter/origin. Our results show that clustering of strong origins located at active promoters can establish early-replicating domains.</p>
dc.identifier.submissionpathoapubs/4456
dc.contributor.departmentProgram in Systems Biology
dc.contributor.departmentDepartment of Biochemistry and Molecular Pharmacology
dc.source.pagese99520


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Copyright 2020 The Authors. Published under the terms of the CC BY NC ND 4.0 license.
Except where otherwise noted, this item's license is described as Copyright 2020 The Authors. Published under the terms of the CC BY NC ND 4.0 license.