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dc.contributor.authorMaldonado-Contreras, Ana
dc.contributor.authorFerrer, Lluis
dc.contributor.authorCawley, Caitlin
dc.contributor.authorCrain, Sarah
dc.contributor.authorBhattarai, Shakti
dc.contributor.authorToscano, Juan
dc.contributor.authorWard, Doyle V.
dc.contributor.authorHoffman, Andrew
dc.date2022-08-11T08:09:57.000
dc.date.accessioned2022-08-23T16:50:30Z
dc.date.available2022-08-23T16:50:30Z
dc.date.issued2020-11-09
dc.date.submitted2021-01-07
dc.identifier.citation<p>Maldonado-Contreras A, Ferrer L, Cawley C, Crain S, Bhattarai S, Toscano J, Ward DV, Hoffman A. Dysbiosis in a canine model of human fistulizing Crohn's disease. Gut Microbes. 2020 Nov 9;12(1):1785246. doi: 10.1080/19490976.2020.1785246. Epub 2020 Jul 30. PMID: 32730134; PMCID: PMC7524328. <a href="https://doi.org/10.1080/19490976.2020.1785246">Link to article on publisher's site</a></p>
dc.identifier.issn1949-0976 (Linking)
dc.identifier.doi10.1080/19490976.2020.1785246
dc.identifier.pmid32730134
dc.identifier.urihttp://hdl.handle.net/20.500.14038/41670
dc.description.abstractCrohn's disease (CD) is a chronic immune-mediated inflammatory condition caused by the loss of mucosal tolerance toward the commensal microbiota. On average, 29.5% and 42.7% CD patients experience perianal complications at 10 and 20 y after diagnosis, respectively. Perianal CD (pCD) result in high disease burden, diminished quality of life, and elevated health-care costs. Overall pCD are predictors of poor long-term outcomes. Animal models of gut inflammation have failed to fully recapitulate the human manifestations of fistulizing CD. Here, we evaluated dogs with spontaneous canine anal furunculosis (CAF), a disease with clinical similarities to pCD, as a surrogate model for understanding the microbial contribution of human pCD pathophysiology. By comparing the gut microbiomes between dogs suffering from CAF (CAF dogs) and healthy dogs, we show CAF-dog microbiomes are either very dissimilar (dysbiotic) or similar (healthy-like), yet unique, to healthy dog's microbiomes. Compared to healthy or healthy-like CAF microbiomes, dysbiotic CAF microbiomes showed an increased abundance of Bacteroides vulgatus and Escherichia coli and a decreased abundance of Megamonas species and Prevotella copri. Our results mirror what have been reported in previous microbiome studies of patients with CD; particularly, CAF dogs exhibited two distinct microbiome composition: dysbiotic and healthy-like, with determinant bacterial taxa such as E. coli and P. copri that overlap what it has been found on their human counterpart. Thus, our results support the use of CAF dogs as a surrogate model to advance our understanding of microbial dynamics in pCD.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=32730134&dopt=Abstract">Link to Article in PubMed</a></p>
dc.rightsCopyright © 2020 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectcanine furunculosis
dc.subjectdysbiosis
dc.subjectfistulizing Crohn’s disease
dc.subjectmicrobiome
dc.subjectperianal fistulas
dc.subjectDigestive System Diseases
dc.subjectDisease Modeling
dc.subjectImmunology and Infectious Disease
dc.subjectMicrobiology
dc.titleDysbiosis in a canine model of human fistulizing Crohn's disease
dc.typeJournal Article
dc.source.journaltitleGut microbes
dc.source.volume12
dc.source.issue1
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=5491&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/4461
dc.identifier.contextkey20969171
refterms.dateFOA2022-08-23T16:50:30Z
html.description.abstract<p>Crohn's disease (CD) is a chronic immune-mediated inflammatory condition caused by the loss of mucosal tolerance toward the commensal microbiota. On average, 29.5% and 42.7% CD patients experience perianal complications at 10 and 20 y after diagnosis, respectively. Perianal CD (pCD) result in high disease burden, diminished quality of life, and elevated health-care costs. Overall pCD are predictors of poor long-term outcomes. Animal models of gut inflammation have failed to fully recapitulate the human manifestations of fistulizing CD. Here, we evaluated dogs with spontaneous canine anal furunculosis (CAF), a disease with clinical similarities to pCD, as a surrogate model for understanding the microbial contribution of human pCD pathophysiology. By comparing the gut microbiomes between dogs suffering from CAF (CAF dogs) and healthy dogs, we show CAF-dog microbiomes are either very dissimilar (dysbiotic) or similar (healthy-like), yet unique, to healthy dog's microbiomes. Compared to healthy or healthy-like CAF microbiomes, dysbiotic CAF microbiomes showed an increased abundance of Bacteroides vulgatus and Escherichia coli and a decreased abundance of Megamonas species and Prevotella copri. Our results mirror what have been reported in previous microbiome studies of patients with CD; particularly, CAF dogs exhibited two distinct microbiome composition: dysbiotic and healthy-like, with determinant bacterial taxa such as E. coli and P. copri that overlap what it has been found on their human counterpart. Thus, our results support the use of CAF dogs as a surrogate model to advance our understanding of microbial dynamics in pCD.</p>
dc.identifier.submissionpathoapubs/4461
dc.contributor.departmentDepartment of Microbiology and Physiological Systems
dc.source.pages1785246


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Copyright © 2020 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Except where otherwise noted, this item's license is described as Copyright © 2020 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.