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dc.contributor.authorChrudinova, Martina
dc.contributor.authorMoreau, Francois
dc.contributor.authorNoh, Hye Lim
dc.contributor.authorPanikova, Terezie
dc.contributor.authorZakova, Lenka
dc.contributor.authorFriedline, Randall H.
dc.contributor.authorValenzuela, Francisco A.
dc.contributor.authorKim, Jason K.
dc.contributor.authorJiracek, Jiri
dc.contributor.authorKahn, C. Ronald
dc.contributor.authorAltindis, Emrah
dc.date2022-08-11T08:09:58.000
dc.date.accessioned2022-08-23T16:50:33Z
dc.date.available2022-08-23T16:50:33Z
dc.date.issued2020-11-19
dc.date.submitted2021-01-07
dc.identifier.citation<p>Chrudinová M, Moreau F, Noh HL, Páníková T, Žáková L, Friedline RH, Valenzuela FA, Kim JK, Jiráček J, Kahn CR, Altindis E. Characterization of viral insulins reveals white adipose tissue-specific effects in mice. Mol Metab. 2020 Nov 19;44:101121. doi: 10.1016/j.molmet.2020.101121. Epub ahead of print. PMID: 33220491; PMCID: PMC7770979. <a href="https://doi.org/10.1016/j.molmet.2020.101121">Link to article on publisher's site</a></p>
dc.identifier.issn2212-8778 (Linking)
dc.identifier.doi10.1016/j.molmet.2020.101121
dc.identifier.pmid33220491
dc.identifier.urihttp://hdl.handle.net/20.500.14038/41681
dc.description<p>This article is based on a previously available preprint on <a href="https://doi.org/10.1101/2020.08.21.261321" target="_blank" title="preprint in bioRxiv">bioRxiv</a> that is also available in <a href="https://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=2789&context=faculty_pubs" target="_blank" title="preprint in eScholarship@UMMS">eScholarship@UMMS</a>.</p>
dc.description.abstractOBJECTIVE: Members of the insulin/insulin-like growth factor (IGF) superfamily are well conserved across the evolutionary tree. We recently showed that four viruses in the Iridoviridae family possess genes that encode proteins highly homologous to human insulin/IGF-1. Using chemically synthesized single-chain (sc), i.e., IGF-1-like, forms of the viral insulin/IGF-1-like peptides (VILPs), we previously showed that they can stimulate human receptors. Because these peptides possess potential cleavage sites to form double chain (dc), i.e., more insulin-like, VILPs, in this study, we have characterized dc forms of VILPs for Grouper iridovirus (GIV), Singapore grouper iridovirus (SGIV) and Lymphocystis disease virus-1 (LCDV-1) for the first time. METHODS: The dcVILPs were chemically synthesized. Using murine fibroblast cell lines overexpressing insulin receptor (IR-A or IR-B) or IGF1R, we first determined the binding affinity of dcVILPs to the receptors and characterized post-receptor signaling. Further, we used C57BL/6J mice to study the effect of dcVILPs on lowering blood glucose. We designed a 3-h dcVILP in vivo infusion experiment to determine the glucose uptake in different tissues. RESULTS: GIV and SGIV dcVILPs bind to both isoforms of human insulin receptor (IR-A and IR-B) and to the IGF1R, and for the latter, show higher affinity than human insulin. These dcVILPs stimulate IR and IGF1R phosphorylation and post-receptor signaling in vitro and in vivo. Both GIV and SGIV dcVILPs stimulate glucose uptake in mice. In vivo infusion experiments revealed that while insulin (0.015 nmol/kg/min) and GIV dcVILP (0.75 nmol/kg/min) stimulated a comparable glucose uptake in heart and skeletal muscle and brown adipose tissue, GIV dcVILP stimulated 2-fold higher glucose uptake in white adipose tissue (WAT) compared to insulin. This was associated with increased Akt phosphorylation and glucose transporter type 4 (GLUT4) gene expression compared to insulin in WAT. CONCLUSIONS: Our results show that GIV and SGIV dcVILPs are active members of the insulin superfamily with unique characteristics. Elucidating the mechanism of tissue specificity for GIV dcVILP will help us to better understand insulin action, design new analogs that specifically target the tissues and provide new insights into their potential role in disease.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=33220491&dopt=Abstract">Link to Article in PubMed</a></p>
dc.rightsCopyright 2020 The Authors. Published by Elsevier GmbH. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAdipose tissue
dc.subjectGLUT4
dc.subjectGlucose metabolism
dc.subjectIGF-1
dc.subjectInsulin
dc.subjectVILPs
dc.subjectViral insulin
dc.subjectViral mimicry
dc.subjectCellular and Molecular Physiology
dc.subjectEndocrinology
dc.subjectEndocrinology, Diabetes, and Metabolism
dc.subjectHormones, Hormone Substitutes, and Hormone Antagonists
dc.subjectMolecular Biology
dc.titleCharacterization of viral insulins reveals white adipose tissue-specific effects in mice
dc.typeJournal Article
dc.source.journaltitleMolecular metabolism
dc.source.volume44
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=5501&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/4471
dc.identifier.contextkey20969182
refterms.dateFOA2022-08-23T16:50:33Z
html.description.abstract<p>OBJECTIVE: Members of the insulin/insulin-like growth factor (IGF) superfamily are well conserved across the evolutionary tree. We recently showed that four viruses in the Iridoviridae family possess genes that encode proteins highly homologous to human insulin/IGF-1. Using chemically synthesized single-chain (sc), i.e., IGF-1-like, forms of the viral insulin/IGF-1-like peptides (VILPs), we previously showed that they can stimulate human receptors. Because these peptides possess potential cleavage sites to form double chain (dc), i.e., more insulin-like, VILPs, in this study, we have characterized dc forms of VILPs for Grouper iridovirus (GIV), Singapore grouper iridovirus (SGIV) and Lymphocystis disease virus-1 (LCDV-1) for the first time.</p> <p>METHODS: The dcVILPs were chemically synthesized. Using murine fibroblast cell lines overexpressing insulin receptor (IR-A or IR-B) or IGF1R, we first determined the binding affinity of dcVILPs to the receptors and characterized post-receptor signaling. Further, we used C57BL/6J mice to study the effect of dcVILPs on lowering blood glucose. We designed a 3-h dcVILP in vivo infusion experiment to determine the glucose uptake in different tissues.</p> <p>RESULTS: GIV and SGIV dcVILPs bind to both isoforms of human insulin receptor (IR-A and IR-B) and to the IGF1R, and for the latter, show higher affinity than human insulin. These dcVILPs stimulate IR and IGF1R phosphorylation and post-receptor signaling in vitro and in vivo. Both GIV and SGIV dcVILPs stimulate glucose uptake in mice. In vivo infusion experiments revealed that while insulin (0.015 nmol/kg/min) and GIV dcVILP (0.75 nmol/kg/min) stimulated a comparable glucose uptake in heart and skeletal muscle and brown adipose tissue, GIV dcVILP stimulated 2-fold higher glucose uptake in white adipose tissue (WAT) compared to insulin. This was associated with increased Akt phosphorylation and glucose transporter type 4 (GLUT4) gene expression compared to insulin in WAT.</p> <p>CONCLUSIONS: Our results show that GIV and SGIV dcVILPs are active members of the insulin superfamily with unique characteristics. Elucidating the mechanism of tissue specificity for GIV dcVILP will help us to better understand insulin action, design new analogs that specifically target the tissues and provide new insights into their potential role in disease.</p>
dc.identifier.submissionpathoapubs/4471
dc.contributor.departmentDivision of Endocrinology, Metabolism and Diabetes, Department of Medicine
dc.contributor.departmentProgram in Molecular Medicine
dc.source.pages101121


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Copyright 2020 The Authors. Published by Elsevier GmbH. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Except where otherwise noted, this item's license is described as Copyright 2020 The Authors. Published by Elsevier GmbH. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).