FLICK: an optimized plate reader-based assay to infer cell death kinetics
Name:
Publisher version
View Source
Access full-text PDFOpen Access
View Source
Check access options
Check access options
Abstract
Evaluating drug sensitivity is improved by directly quantifying death kinetics, rather than correlates of viability, such as metabolic activity. This is challenging, requiring time-lapse microscopy and genetically encoded labels to distinguish live and dead cells. Here, we describe fluorescence-based and lysis-dependent inference of cell death kinetics (FLICK). This method requires only a standard fluorescence plate reader, retaining the high-throughput nature and broad accessibility of common viability assays. However, FLICK specifically quantifies death, including an accurate inference of death kinetics. For complete details on the use and execution of this protocol, please refer to Richards et al. (2020).Source
Richards R, Honeywell ME, Lee MJ. FLICK: an optimized plate reader-based assay to infer cell death kinetics. STAR Protoc. 2021 Feb 3;2(1):100327. doi: 10.1016/j.xpro.2021.100327. PMID: 33659903; PMCID: PMC7890003. Link to article on publisher's site
DOI
10.1016/j.xpro.2021.100327Permanent Link to this Item
http://hdl.handle.net/20.500.14038/41822PubMed ID
33659903Related Resources
Rights
Copyright 2021 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).Distribution License
http://creativecommons.org/licenses/by-nc-nd/4.0/ae974a485f413a2113503eed53cd6c53
10.1016/j.xpro.2021.100327
Scopus Count
Except where otherwise noted, this item's license is described as Copyright 2021 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).