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dc.contributor.authorRichards, Ryan
dc.contributor.authorHoneywell, Megan E
dc.contributor.authorLee, Michael J
dc.date2022-08-11T08:09:59.000
dc.date.accessioned2022-08-23T16:51:15Z
dc.date.available2022-08-23T16:51:15Z
dc.date.issued2021-02-03
dc.date.submitted2021-05-28
dc.identifier.citation<p>Richards R, Honeywell ME, Lee MJ. FLICK: an optimized plate reader-based assay to infer cell death kinetics. STAR Protoc. 2021 Feb 3;2(1):100327. doi: 10.1016/j.xpro.2021.100327. PMID: 33659903; PMCID: PMC7890003. <a href="https://doi.org/10.1016/j.xpro.2021.100327">Link to article on publisher's site</a></p>
dc.identifier.issn2666-1667 (Linking)
dc.identifier.doi10.1016/j.xpro.2021.100327
dc.identifier.pmid33659903
dc.identifier.urihttp://hdl.handle.net/20.500.14038/41822
dc.description.abstractEvaluating drug sensitivity is improved by directly quantifying death kinetics, rather than correlates of viability, such as metabolic activity. This is challenging, requiring time-lapse microscopy and genetically encoded labels to distinguish live and dead cells. Here, we describe fluorescence-based and lysis-dependent inference of cell death kinetics (FLICK). This method requires only a standard fluorescence plate reader, retaining the high-throughput nature and broad accessibility of common viability assays. However, FLICK specifically quantifies death, including an accurate inference of death kinetics. For complete details on the use and execution of this protocol, please refer to Richards et al. (2020).
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=33659903&dopt=Abstract">Link to Article in PubMed</a></p>
dc.rightsCopyright 2021 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCancer
dc.subjectCell-based assays
dc.subjectHigh-throughput screening
dc.subjectCancer Biology
dc.subjectCell Biology
dc.subjectSystems Biology
dc.titleFLICK: an optimized plate reader-based assay to infer cell death kinetics
dc.typeJournal Article
dc.source.journaltitleSTAR protocols
dc.source.volume2
dc.source.issue1
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=5653&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/4622
dc.identifier.contextkey23121442
refterms.dateFOA2022-08-23T16:51:16Z
html.description.abstract<p>Evaluating drug sensitivity is improved by directly quantifying death kinetics, rather than correlates of viability, such as metabolic activity. This is challenging, requiring time-lapse microscopy and genetically encoded labels to distinguish live and dead cells. Here, we describe fluorescence-based and lysis-dependent inference of cell death kinetics (FLICK). This method requires only a standard fluorescence plate reader, retaining the high-throughput nature and broad accessibility of common viability assays. However, FLICK specifically quantifies death, including an accurate inference of death kinetics. For complete details on the use and execution of this protocol, please refer to Richards et al. (2020).</p>
dc.identifier.submissionpathoapubs/4622
dc.contributor.departmentGraduate School of Biomedical Sciences
dc.contributor.departmentProgram in Systems Biology
dc.source.pages100327


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Copyright 2021 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Except where otherwise noted, this item's license is described as Copyright 2021 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).