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dc.contributor.authorOzcan, Ezgi
dc.contributor.authorRozycki, Michelle R.
dc.contributor.authorSela, David A.
dc.date2022-08-11T08:09:59.000
dc.date.accessioned2022-08-23T16:51:22Z
dc.date.available2022-08-23T16:51:22Z
dc.date.issued2021-03-22
dc.date.submitted2021-06-14
dc.identifier.citation<p>Özcan E, Rozycki MR, Sela DA. Cranberry Proanthocyanidins and Dietary Oligosaccharides Synergistically Modulate <em>Lactobacillus plantarum</em> Physiology. Microorganisms. 2021 Mar 22;9(3):656. doi: 10.3390/microorganisms9030656. PMID: 33810188; PMCID: PMC8004764. <a href="https://doi.org/10.3390/microorganisms9030656">Link to article on publisher's site</a></p>
dc.identifier.issn2076-2607 (Linking)
dc.identifier.doi10.3390/microorganisms9030656
dc.identifier.pmid33810188
dc.identifier.urihttp://hdl.handle.net/20.500.14038/41845
dc.description.abstractPlant-based foods contain bioactive compounds such as polyphenols that resist digestion and potentially benefit the host through interactions with their resident microbiota. Based on previous observations, we hypothesized that the probiotic Lactobacillus plantarum interacts with cranberry polyphenols and dietary oligosaccharides to synergistically impact its physiology. In this study, L. plantarum ATCC BAA-793 was grown on dietary oligosaccharides, including cranberry xyloglucans, fructooligosaccharides, and human milk oligosaccharides, in conjunction with proanthocyanidins (PACs) extracted from cranberries. As a result, L. plantarum exhibits a differential physiological response to cranberry PACs dependent on the carbohydrate source and polyphenol fraction introduced. Of the two PAC extracts evaluated, the PAC1 fraction contains higher concentrations of PACs and increased growth regardless of the oligosaccharide, whereas PAC2 positively modulates its growth during xyloglucan metabolism. Interestingly, fructooligosaccharides (FOS) are efficiently utilized in the presence of PAC1, as this L. plantarum strain does not utilize this substrate typically. Relative to glucose, oligosaccharide metabolism increases the ratio of secreted acetic acid to lactic acid. The PAC2 fraction differentially increases this ratio during cranberry xyloglucan fermentation compared with PAC1. The global transcriptome links the expression of putative polyphenol degradation genes and networks and metabolic phenotypes.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=33810188&dopt=Abstract">Link to Article in PubMed</a></p>
dc.rightsCopyright © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectcranberry
dc.subjectlactobacillus
dc.subjectnutrition
dc.subjectoligosaccharides
dc.subjectpolyphenols
dc.subjectprobiotics
dc.subjectBacteria
dc.subjectMicrobiology
dc.titleCranberry Proanthocyanidins and Dietary Oligosaccharides Synergistically Modulate Lactobacillus plantarum Physiology
dc.typeJournal Article
dc.source.journaltitleMicroorganisms
dc.source.volume9
dc.source.issue3
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=5679&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/4648
dc.identifier.contextkey23344519
refterms.dateFOA2022-08-23T16:51:22Z
html.description.abstract<p>Plant-based foods contain bioactive compounds such as polyphenols that resist digestion and potentially benefit the host through interactions with their resident microbiota. Based on previous observations, we hypothesized that the probiotic Lactobacillus plantarum interacts with cranberry polyphenols and dietary oligosaccharides to synergistically impact its physiology. In this study, L. plantarum ATCC BAA-793 was grown on dietary oligosaccharides, including cranberry xyloglucans, fructooligosaccharides, and human milk oligosaccharides, in conjunction with proanthocyanidins (PACs) extracted from cranberries. As a result, L. plantarum exhibits a differential physiological response to cranberry PACs dependent on the carbohydrate source and polyphenol fraction introduced. Of the two PAC extracts evaluated, the PAC1 fraction contains higher concentrations of PACs and increased growth regardless of the oligosaccharide, whereas PAC2 positively modulates its growth during xyloglucan metabolism. Interestingly, fructooligosaccharides (FOS) are efficiently utilized in the presence of PAC1, as this L. plantarum strain does not utilize this substrate typically. Relative to glucose, oligosaccharide metabolism increases the ratio of secreted acetic acid to lactic acid. The PAC2 fraction differentially increases this ratio during cranberry xyloglucan fermentation compared with PAC1. The global transcriptome links the expression of putative polyphenol degradation genes and networks and metabolic phenotypes.</p>
dc.identifier.submissionpathoapubs/4648
dc.contributor.departmentDepartment of Microbiology and Physiological Systems
dc.source.pages656


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Copyright © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Except where otherwise noted, this item's license is described as Copyright © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).