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    A transgenic mouse model for studying the clearance of blood-borne pathogens via human complement receptor 1 (CR1)

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    Authors
    Repik, Alexander
    Pincus, Steve E.
    Ghiran, Ionita
    Nicholson-Weller, Anne
    Asher, Damon R.
    Cerny, Anna M.
    Casey, Leslie S.
    Jones, S. M.
    Jones, Stephen N.
    Mohamed, Nehal
    Klickstein, Lloyd B.
    Spitalny, George L.
    Finberg, Robert W.
    Show allShow less
    UMass Chan Affiliations
    Program in Molecular Medicine
    Department of Medicine
    Document Type
    Journal Article
    Publication Date
    2005-04-06
    Keywords
    Animals
    Antibodies, Bispecific
    Antigen-Antibody Complex
    Bacteriophage phi X 174
    *Blood-Borne Pathogens
    *Disease Models, Animal
    Dose-Response Relationship, Immunologic
    Erythrocytes
    Humans
    Mice
    Mice, Inbred C57BL
    Mice, Transgenic
    Microscopy, Fluorescence
    Papio
    Receptors, Complement
    Immunology and Infectious Disease
    Show allShow less
    
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    Link to Full Text
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1809366/
    Abstract
    Complement receptor 1 (CR1) on the surface of human erythrocytes facilitates intravascular clearance of complement-opsonized pathogens. The need for complement activation can be circumvented by directly coupling the organism to CR1 using a bispecific monoclonal antibody heteropolymer (HP). Lack of a functional homologue to CR1 on mouse erythrocytes has made it difficult to study HP-dependent clearance of pathogens in small animals. We have developed a transgenic mouse that expresses human CR1 on erythrocytes. CR1 antigen is of appropriate size and in a clustered distribution as confirmed by immunoblotting and fluorescence microscopy, respectively. HP that immobilized bacteriophage PhiX174 prototype pathogen to erythrocyte CR1 of the transgenic mice increased the rate of clearance of the virus compared with HP that bound bacteriophage, but not CR1. This transgenic mouse model will allow evaluation of different HPs for their in vivo efficacy and potential as human therapeutics.
    Source

    Clin Exp Immunol. 2005 May;140(2):230-40. Link to article on publisher's site

    DOI
    10.1111/j.1365-2249.2005.02764
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/41865
    PubMed ID
    15807846
    Related Resources

    Link to article in PubMed

    ae974a485f413a2113503eed53cd6c53
    10.1111/j.1365-2249.2005.02764
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