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Histological assessment of developmental cell death in Drosophila pupae

dc.contributor.authorVelentzas, Panagiotis D.
dc.contributor.authorBaehrecke, Eric H.
dc.date2022-08-11T08:10:00.000
dc.date.accessioned2022-08-23T16:51:41Z
dc.date.available2022-08-23T16:51:41Z
dc.date.issued2021-04-20
dc.date.submitted2021-08-12
dc.identifier.citation<p>Velentzas PD, Baehrecke EH. Histological assessment of developmental cell death in <em>Drosophila</em> pupae. STAR Protoc. 2021 Apr 20;2(2):100473. doi: 10.1016/j.xpro.2021.100473. PMID: 33997806; PMCID: PMC8091131. <a href="https://doi.org/10.1016/j.xpro.2021.100473">Link to article on publisher's site</a></p>
dc.identifier.issn2666-1667 (Linking)
dc.identifier.doi10.1016/j.xpro.2021.100473
dc.identifier.pmid33997806
dc.identifier.urihttp://hdl.handle.net/20.500.14038/41905
dc.description.abstractThis protocol describes the embedding and processing of Drosophila pupae in paraffin to monitor tissue changes during development. Although multiple methods are available to evaluate developmental changes in Drosophila embryos, imaging detailed changes during metamorphosis is challenging as the animal is enclosed in the cuticle, rendering it inaccessible to whole mount imaging. Here, we present a protocol that focuses on developmental clearance of the larval salivary glands in Drosophila pupae that can be extended to examine other tissues/stages for similar purposes. For complete details on the use and execution of this protocol, please refer to Velentzas et al. (2018).
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=33997806&dopt=Abstract">Link to Article in PubMed</a></p>
dc.rightsCopyright 2021 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCell Biology
dc.subjectDevelopmental biology
dc.subjectMicroscopy
dc.subjectModel Organisms
dc.subjectCell Biology
dc.subjectDevelopmental Biology
dc.subjectResearch Methods in Life Sciences
dc.titleHistological assessment of developmental cell death in Drosophila pupae
dc.typeJournal Article
dc.source.journaltitleSTAR protocols
dc.source.volume2
dc.source.issue2
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=5745&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/4712
dc.identifier.contextkey24296661
refterms.dateFOA2022-08-23T16:51:41Z
html.description.abstract<p>This protocol describes the embedding and processing of Drosophila pupae in paraffin to monitor tissue changes during development. Although multiple methods are available to evaluate developmental changes in Drosophila embryos, imaging detailed changes during metamorphosis is challenging as the animal is enclosed in the cuticle, rendering it inaccessible to whole mount imaging. Here, we present a protocol that focuses on developmental clearance of the larval salivary glands in Drosophila pupae that can be extended to examine other tissues/stages for similar purposes. For complete details on the use and execution of this protocol, please refer to Velentzas et al. (2018).</p>
dc.identifier.submissionpathoapubs/4712
dc.contributor.departmentDepartment of Molecular, Cell and Cancer Biology
dc.source.pages100473


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Copyright 2021 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Except where otherwise noted, this item's license is described as Copyright 2021 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).