Show simple item record

dc.contributor.authorMarks, Sandy C. Jr.
dc.contributor.authorLundmark, Carin
dc.contributor.authorWurtz, Tilmann
dc.contributor.authorOdgren, Paul R.
dc.contributor.authorMacKay, Carole A.
dc.contributor.authorMason-Savas, April
dc.contributor.authorPopoff, Steven N.
dc.date2022-08-11T08:10:00.000
dc.date.accessioned2022-08-23T16:51:50Z
dc.date.available2022-08-23T16:51:50Z
dc.date.issued1999-06-18
dc.date.submitted2008-07-09
dc.identifier.citation<p>Dev Dyn. 1999 Jun;215(2):117-25. <a>3.0.CO;2-D" >Link to article on publisher's site</a></p>
dc.identifier.issn1058-8388 (Print)
dc.identifier.doi10.1002/(SICI)1097-0177(199906)215:2<117::AID-DVDY4>3.0.CO;2-D
dc.identifier.pmid10373016
dc.identifier.urihttp://hdl.handle.net/20.500.14038/41935
dc.description.abstractThe toothless (osteopetrotic) mutation in the rat is characterized by retarded development of the anterior facial skeleton. Growth of the anterior face in rats occurs at the premaxillary-maxillary suture (PMMS). To identify potential mechanisms for stunted facial growth in this mutation we compared the temporospatial expression of collagen I (Col I) and collagen III (Col III) RNA around this suture in toothless (tl) rats and normal littermates by in situ hybridization of specific riboprobes in sagittal sections of the head. In normal rats, the suture is S shaped at birth and becomes highly convoluted by 10 days with cells in the center (fibroblasts and osteoblast progenitors) expressing Col III RNA and those at the periphery (osteoblasts) expressing no Col III RNA but high amounts of Col I RNA throughout the growth phase (the first 2 postnatal weeks). In the mutant PMMS, cells were reduced in number, less differentiated, and fewer osteoblasts were encountered. Expression of Col I RNA was at normal levels, but centrosutural cells expressed Col III RNA only after day 6 and then only weakly. A highly convoluted sutural shape was never achieved in mutants during the first 2 postnatal weeks. Treatment of tl rats with the cytokine CSF-1 improved facial growth and restored cellular diversity and Col III RNA expression in the PMMS to normal levels. Taken together, these data suggest that normal facial growth in rats is related to expression of Col III RNAby osteoblast precursors in the PMMS, that these cells are deficient in the tl mutation and are rescued following treatment with CSF-1.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=10373016&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.1002/(SICI)1097-0177(199906)215:2<117::AID-DVDY4>3.0.CO;2-D
dc.subjectAnimals
dc.subjectCollagen
dc.subject*Gene Expression Regulation, Developmental
dc.subjectMacrophage Colony-Stimulating Factor
dc.subjectMaxillofacial Development
dc.subjectOsteopetrosis
dc.subjectRNA
dc.subjectRats
dc.subjectRats, Mutant Strains
dc.subjectCell Biology
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleFacial development and type III collagen RNA expression: concurrent repression in the osteopetrotic (Toothless,tl) rat and rescue after treatment with colony-stimulating factor-1
dc.typeJournal Article
dc.source.journaltitleDevelopmental dynamics : an official publication of the American Association of Anatomists
dc.source.volume215
dc.source.issue2
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/474
dc.identifier.contextkey544956
html.description.abstract<p>The toothless (osteopetrotic) mutation in the rat is characterized by retarded development of the anterior facial skeleton. Growth of the anterior face in rats occurs at the premaxillary-maxillary suture (PMMS). To identify potential mechanisms for stunted facial growth in this mutation we compared the temporospatial expression of collagen I (Col I) and collagen III (Col III) RNA around this suture in toothless (tl) rats and normal littermates by in situ hybridization of specific riboprobes in sagittal sections of the head. In normal rats, the suture is S shaped at birth and becomes highly convoluted by 10 days with cells in the center (fibroblasts and osteoblast progenitors) expressing Col III RNA and those at the periphery (osteoblasts) expressing no Col III RNA but high amounts of Col I RNA throughout the growth phase (the first 2 postnatal weeks). In the mutant PMMS, cells were reduced in number, less differentiated, and fewer osteoblasts were encountered. Expression of Col I RNA was at normal levels, but centrosutural cells expressed Col III RNA only after day 6 and then only weakly. A highly convoluted sutural shape was never achieved in mutants during the first 2 postnatal weeks. Treatment of tl rats with the cytokine CSF-1 improved facial growth and restored cellular diversity and Col III RNA expression in the PMMS to normal levels. Taken together, these data suggest that normal facial growth in rats is related to expression of Col III RNAby osteoblast precursors in the PMMS, that these cells are deficient in the tl mutation and are rescued following treatment with CSF-1.</p>
dc.identifier.submissionpathoapubs/474
dc.contributor.departmentDepartment of Cell Biology
dc.source.pages117-25


This item appears in the following Collection(s)

Show simple item record