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dc.contributor.authorWaldemar, Racki J.
dc.contributor.authorRichter, Joel D.
dc.date2022-08-11T08:10:00.000
dc.date.accessioned2022-08-23T16:51:53Z
dc.date.available2022-08-23T16:51:53Z
dc.date.issued2006-10-20
dc.date.submitted2008-07-09
dc.identifier.citationDevelopment. 2006 Nov;133(22):4527-37. Epub 2006 Oct 18. <a href="http://dx.doi.org/10.1242/dev.02651">Link to article on publisher's site</a>
dc.identifier.issn0950-1991 (Print)
dc.identifier.doi10.1242/dev.02651
dc.identifier.pmid17050619
dc.identifier.urihttp://hdl.handle.net/20.500.14038/41945
dc.description.abstractCPEB is a sequence-specific RNA-binding protein that regulates polyadenylation-induced translation. In Cpeb knockout mice, meiotic progression is disrupted at pachytene due to inhibited translation of synaptonemal complex protein mRNAs. To assess the function of CPEB after pachytene, we used the zona pellucida 3 (Zp3) promoter to generate transgenic mice expressing siRNA that induce the destruction of Cpeb mRNA. Oocytes from these animals do not develop normally; they undergo parthenogenetic cell division in the ovary, exhibit abnormal polar bodies, are detached from the cumulus granulosa cell layer, and display spindle and nuclear anomalies. In addition, many follicles contain apoptotic granulosa cells. CPEB binds several oocyte mRNAs, including Smad1, Smad5, spindlin, Bub1b, Mos, H1foo, Obox1, Dnmt1o, TiParp, Trim61 and Gdf9, a well described oocyte-expressed growth factor that is necessary for follicle development. In Cpeb knockdown oocytes, Gdf9 RNA has a shortened poly(A) tail and reduced expression. These data indicate that CPEB controls the expression of Gdf9 mRNA, which in turn is necessary for oocyte-follicle development. Finally, several phenotypes, i.e. progressive oocyte loss and infertility, elicited by the knockdown of CPEB in oocytes resemble those of the human premature ovarian failure syndrome.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=17050619&dopt=Abstract">Link to Article in PubMed</a>
dc.subjectAnimals
dc.subjectBlotting, Western
dc.subjectDNA Primers
dc.subjectFemale
dc.subjectIntercellular Signaling Peptides and Proteins
dc.subjectMice
dc.subjectMice, Transgenic
dc.subjectModels, Biological
dc.subjectOocytes
dc.subjectOvarian Follicle
dc.subjectRNA, Messenger
dc.subjectRNA, Small Interfering
dc.subjectRNA-Binding Proteins
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectEndocrine System Diseases
dc.subjectEndocrinology, Diabetes, and Metabolism
dc.subjectMolecular Biology
dc.titleCPEB controls oocyte growth and follicle development in the mouse
dc.typeJournal Article
dc.source.journaltitleDevelopment (Cambridge, England)
dc.source.volume133
dc.source.issue22
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1474&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/475
dc.identifier.contextkey544957
refterms.dateFOA2022-08-23T16:51:53Z
html.description.abstract<p>CPEB is a sequence-specific RNA-binding protein that regulates polyadenylation-induced translation. In Cpeb knockout mice, meiotic progression is disrupted at pachytene due to inhibited translation of synaptonemal complex protein mRNAs. To assess the function of CPEB after pachytene, we used the zona pellucida 3 (Zp3) promoter to generate transgenic mice expressing siRNA that induce the destruction of Cpeb mRNA. Oocytes from these animals do not develop normally; they undergo parthenogenetic cell division in the ovary, exhibit abnormal polar bodies, are detached from the cumulus granulosa cell layer, and display spindle and nuclear anomalies. In addition, many follicles contain apoptotic granulosa cells. CPEB binds several oocyte mRNAs, including Smad1, Smad5, spindlin, Bub1b, Mos, H1foo, Obox1, Dnmt1o, TiParp, Trim61 and Gdf9, a well described oocyte-expressed growth factor that is necessary for follicle development. In Cpeb knockdown oocytes, Gdf9 RNA has a shortened poly(A) tail and reduced expression. These data indicate that CPEB controls the expression of Gdf9 mRNA, which in turn is necessary for oocyte-follicle development. Finally, several phenotypes, i.e. progressive oocyte loss and infertility, elicited by the knockdown of CPEB in oocytes resemble those of the human premature ovarian failure syndrome.</p>
dc.identifier.submissionpathoapubs/475
dc.contributor.departmentDepartment of Medicine, Division of Diabetes
dc.contributor.departmentProgram in Molecular Medicine
dc.source.pages4527-37


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