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dc.contributor.authorGanguly, Atish
dc.contributor.authorJiang, Jin
dc.contributor.authorIp, Y. Tony
dc.date2022-08-11T08:10:00.000
dc.date.accessioned2022-08-23T16:51:56Z
dc.date.available2022-08-23T16:51:56Z
dc.date.issued2005-07-01
dc.date.submitted2008-07-09
dc.identifier.citationDevelopment. 2005 Aug;132(15):3419-29. Epub 2005 Jun 29. <a href="http://dx.doi.org/10.1242/dev.01903">Link to article on publisher's site</a>
dc.identifier.issn0950-1991 (Print)
dc.identifier.doi10.1242/dev.01903
dc.identifier.pmid15987775
dc.identifier.urihttp://hdl.handle.net/20.500.14038/41955
dc.description.abstractThe maternal Toll signaling pathway sets up a nuclear gradient of the transcription factor Dorsal in the early Drosophila embryo. Dorsal activates twist and snail, and the Dorsal/Twist/Snail network activates and represses other zygotic genes to form the correct expression patterns along the dorsoventral axis. An essential function of this patterning is to promote ventral cell invagination during mesoderm formation, but how the downstream genes regulate ventral invagination is not known. We show here that wntD is a novel member of the Wnt family. The expression of wntD is activated by Dorsal and Twist, but the expression is much reduced in the ventral cells through repression by Snail. Overexpression of WntD in the early embryo inhibits ventral invagination, suggesting that the de-repressed WntD in snail mutant embryos may contribute to inhibiting ventral invagination. The overexpressed WntD inhibits invagination by antagonizing Dorsal nuclear localization, as well as twist and snail expression. Consistent with the early expression of WntD at the poles in wild-type embryos, loss of WntD leads to posterior expansion of nuclear Dorsal and snail expression, demonstrating that physiological levels of WntD can also attenuate Dorsal nuclear localization. We also show that the de-repressed WntD in snail mutant embryos contributes to the premature loss of snail expression, probably by inhibiting Dorsal. Thus, these results together demonstrate that WntD is regulated by the Dorsal/Twist/Snail network, and is an inhibitor of Dorsal nuclear localization and function.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=15987775&dopt=Abstract">Link to Article in PubMed</a>
dc.subjectAmino Acid Sequence
dc.subjectAnimals
dc.subjectBody Patterning
dc.subjectDrosophila Proteins
dc.subjectDrosophila melanogaster
dc.subjectEmbryo, Nonmammalian
dc.subjectGastrula
dc.subjectIntracellular Signaling Peptides and Proteins
dc.subjectMolecular Sequence Data
dc.subjectMorphogenesis
dc.subjectNuclear Proteins
dc.subjectPhosphoproteins
dc.subjectSequence Alignment
dc.subjectSequence Homology, Amino Acid
dc.subjectTranscription Factors
dc.subjectTwist Transcription Factor
dc.subjectZinc Fingers
dc.subjectBiochemistry, Biophysics, and Structural Biology
dc.subjectMolecular Biology
dc.titleDrosophila WntD is a target and an inhibitor of the Dorsal/Twist/Snail network in the gastrulating embryo
dc.typeJournal Article
dc.source.journaltitleDevelopment (Cambridge, England)
dc.source.volume132
dc.source.issue15
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1475&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/476
dc.identifier.contextkey544958
refterms.dateFOA2022-08-23T16:51:57Z
html.description.abstract<p>The maternal Toll signaling pathway sets up a nuclear gradient of the transcription factor Dorsal in the early Drosophila embryo. Dorsal activates twist and snail, and the Dorsal/Twist/Snail network activates and represses other zygotic genes to form the correct expression patterns along the dorsoventral axis. An essential function of this patterning is to promote ventral cell invagination during mesoderm formation, but how the downstream genes regulate ventral invagination is not known. We show here that wntD is a novel member of the Wnt family. The expression of wntD is activated by Dorsal and Twist, but the expression is much reduced in the ventral cells through repression by Snail. Overexpression of WntD in the early embryo inhibits ventral invagination, suggesting that the de-repressed WntD in snail mutant embryos may contribute to inhibiting ventral invagination. The overexpressed WntD inhibits invagination by antagonizing Dorsal nuclear localization, as well as twist and snail expression. Consistent with the early expression of WntD at the poles in wild-type embryos, loss of WntD leads to posterior expansion of nuclear Dorsal and snail expression, demonstrating that physiological levels of WntD can also attenuate Dorsal nuclear localization. We also show that the de-repressed WntD in snail mutant embryos contributes to the premature loss of snail expression, probably by inhibiting Dorsal. Thus, these results together demonstrate that WntD is regulated by the Dorsal/Twist/Snail network, and is an inhibitor of Dorsal nuclear localization and function.</p>
dc.identifier.submissionpathoapubs/476
dc.contributor.departmentProgram in Molecular Medicine
dc.contributor.departmentGraduate School of Biomedical Sciences
dc.source.pages3419-29


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