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dc.contributor.authorZhang, Jinhua
dc.contributor.authorJin, Zhe
dc.contributor.authorBao, Zheng-Zheng
dc.date2022-08-11T08:10:00.000
dc.date.accessioned2022-08-23T16:52:00Z
dc.date.available2022-08-23T16:52:00Z
dc.date.issued2004-02-27
dc.date.submitted2008-07-09
dc.identifier.citationDevelopment. 2004 Apr;131(7):1553-62. Epub 2004 Feb 25. <a href="http://dx.doi.org/10.1242/dev.01041">Link to article on publisher's site</a>
dc.identifier.issn0950-1991 (Print)
dc.identifier.doi10.1242/dev.01041
dc.identifier.pmid14985256
dc.identifier.urihttp://hdl.handle.net/20.500.14038/41966
dc.description.abstractThe targeting of retinal ganglion axons toward the optic disc is the first step in axon pathfinding in the visual system. The molecular mechanisms involved in guiding the retinal axons to project towards the optic disc are not well understood. We report that a gene encoding a zinc-finger transcription factor, Zic3, is expressed in a periphery-high and center-low gradient in the retina at the stages of active axon extension inside the retina. The gradient expression of Zic3 recedes towards the periphery over the course of development, correlating with the progression of retinal cell differentiation and axonogenesis. Disruption of gradient expression of Zic3 by retroviral overexpression resulted in mis-targeting of retinal axons and some axons misrouted to the sub-retinal space at the photoreceptor side of the retina. Misexpression of Zic3 did not affect neurogenesis or differentiation inside the retina, or grossly alter retinal lamination. By stripe assay, we show that misexpression of Zic3 may induce the expression of an inhibitory factor to the retinal axons. Zic3 appears to play a role in intra-retinal axon targeting, possibly through regulation of the expression of specific downstream genes involved in axon guidance.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=14985256&dopt=Abstract">Link to Article in PubMed</a>
dc.subjectAnimals
dc.subjectAxons
dc.subjectCell Differentiation
dc.subjectChick Embryo
dc.subjectGene Expression Regulation, Developmental
dc.subjectHomeodomain Proteins
dc.subjectHumans
dc.subjectIn Situ Hybridization
dc.subjectIn Situ Nick-End Labeling
dc.subject*Morphogenesis
dc.subjectNerve Growth Factors
dc.subjectPhenotype
dc.subjectRetina
dc.subjectRetinal Ganglion Cells
dc.subjectTranscription Factors
dc.subjectTumor Suppressor Proteins
dc.subjectCell Biology
dc.subjectEndocrinology, Diabetes, and Metabolism
dc.titleDisruption of gradient expression of Zic3 resulted in abnormal intra-retinal axon projection
dc.typeJournal Article
dc.source.journaltitleDevelopment (Cambridge, England)
dc.source.volume131
dc.source.issue7
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1476&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/477
dc.identifier.contextkey544959
refterms.dateFOA2022-08-23T16:52:00Z
html.description.abstract<p>The targeting of retinal ganglion axons toward the optic disc is the first step in axon pathfinding in the visual system. The molecular mechanisms involved in guiding the retinal axons to project towards the optic disc are not well understood. We report that a gene encoding a zinc-finger transcription factor, Zic3, is expressed in a periphery-high and center-low gradient in the retina at the stages of active axon extension inside the retina. The gradient expression of Zic3 recedes towards the periphery over the course of development, correlating with the progression of retinal cell differentiation and axonogenesis. Disruption of gradient expression of Zic3 by retroviral overexpression resulted in mis-targeting of retinal axons and some axons misrouted to the sub-retinal space at the photoreceptor side of the retina. Misexpression of Zic3 did not affect neurogenesis or differentiation inside the retina, or grossly alter retinal lamination. By stripe assay, we show that misexpression of Zic3 may induce the expression of an inhibitory factor to the retinal axons. Zic3 appears to play a role in intra-retinal axon targeting, possibly through regulation of the expression of specific downstream genes involved in axon guidance.</p>
dc.identifier.submissionpathoapubs/477
dc.contributor.departmentDepartment of Medicine, Division of Endocrinology and Metabolism and Cell Biology
dc.source.pages1553-62


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