Authors
He, LianHuang, Zixian
Huang, Kai
Chen, Rui
Nguyen, Nhung T.
Wang, Rui
Cai, Xiaoli
Huang, Zhiquan
Siwko, Stefan
Walker, Joel R.
Han, Gang
Zhou, Yubin
Jing, Ji
UMass Chan Affiliations
Department of Biochemistry and Molecular PharmacologyDocument Type
Journal ArticlePublication Date
2021-05-06Keywords
bioluminescencenecroptosis
optogenetics
pyroptosis
upconversion nanoparticles
Cancer Biology
Cell Biology
Metadata
Show full item recordAbstract
Herein, a set of optogenetic tools (designated LiPOP) that enable photoswitchable necroptosis and pyroptosis in live cells with varying kinetics, is introduced. The LiPOP tools allow reconstruction of the key molecular steps involved in these two non-apoptotic cell death pathways by harnessing the power of light. Further, the use of LiPOPs coupled with upconversion nanoparticles or bioluminescence is demonstrated to achieve wireless optogenetic or chemo-optogenetic killing of cancer cells in multiple mouse tumor models. LiPOPs can trigger necroptotic and pyroptotic cell death in cultured prokaryotic or eukaryotic cells and in living animals, and set the stage for studying the role of non-apoptotic cell death pathways during microbial infection and anti-tumor immunity.Source
He L, Huang Z, Huang K, Chen R, Nguyen NT, Wang R, Cai X, Huang Z, Siwko S, Walker JR, Han G, Zhou Y, Jing J. Optogenetic Control of Non-Apoptotic Cell Death. Adv Sci (Weinh). 2021 May 6;8(13):2100424. doi: 10.1002/advs.202100424. PMID: 34540558; PMCID: PMC8438606. Link to article on publisher's site
DOI
10.1002/advs.202100424Permanent Link to this Item
http://hdl.handle.net/20.500.14038/42019PubMed ID
34540558Related Resources
Rights
Copyright © 2021 The Authors. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.Distribution License
http://creativecommons.org/licenses/by/4.0/ae974a485f413a2113503eed53cd6c53
10.1002/advs.202100424
Scopus Count
Collections
Except where otherwise noted, this item's license is described as Copyright © 2021 The Authors. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.