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dc.contributor.authorChoppakatla, Pavan
dc.contributor.authorDekker, Bastiaan
dc.contributor.authorCutts, Erin E.
dc.contributor.authorVannini, Alessandro
dc.contributor.authorDekker, Job
dc.contributor.authorFunabiki, Hironori
dc.date2022-08-11T08:10:01.000
dc.date.accessioned2022-08-23T16:52:35Z
dc.date.available2022-08-23T16:52:35Z
dc.date.issued2021-08-18
dc.date.submitted2022-02-28
dc.identifier.citation<p>Choppakatla P, Dekker B, Cutts EE, Vannini A, Dekker J, Funabiki H. Linker histone H1.8 inhibits chromatin binding of condensins and DNA topoisomerase II to tune chromosome length and individualization. Elife. 2021 Aug 18;10:e68918. doi: 10.7554/eLife.68918. PMID: 34406118; PMCID: PMC8416026. <a href="https://doi.org/10.7554/eLife.68918">Link to article on publisher's site</a></p>
dc.identifier.issn2050-084X (Linking)
dc.identifier.doi10.7554/eLife.68918
dc.identifier.pmid34406118
dc.identifier.urihttp://hdl.handle.net/20.500.14038/42081
dc.description.abstractDNA loop extrusion by condensins and decatenation by DNA topoisomerase II (topo II) are thought to drive mitotic chromosome compaction and individualization. Here, we reveal that the linker histone H1.8 antagonizes condensins and topo II to shape mitotic chromosome organization. In vitro chromatin reconstitution experiments demonstrate that H1.8 inhibits binding of condensins and topo II to nucleosome arrays. Accordingly, H1.8 depletion in Xenopus egg extracts increased condensins and topo II levels on mitotic chromatin. Chromosome morphology and Hi-C analyses suggest that H1.8 depletion makes chromosomes thinner and longer through shortening the average loop size and reducing the DNA amount in each layer of mitotic loops. Furthermore, excess loading of condensins and topo II to chromosomes by H1.8 depletion causes hyper-chromosome individualization and dispersion. We propose that condensins and topo II are essential for chromosome individualization, but their functions are tuned by the linker histone to keep chromosomes together until anaphase.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=34406118&dopt=Abstract">Link to Article in PubMed</a></p>
dc.rightsCopyright © 2021, Choppakatla et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectHi-C
dc.subjectchromatin
dc.subjectchromosome compaction
dc.subjectchromosomes
dc.subjectgene expression
dc.subjectlinker histone
dc.subjectmitosis
dc.subjectnucleosome
dc.subjectxenopus
dc.subjectBiochemistry, Biophysics, and Structural Biology
dc.subjectCell and Developmental Biology
dc.titleLinker histone H1.8 inhibits chromatin binding of condensins and DNA topoisomerase II to tune chromosome length and individualization
dc.typeJournal Article
dc.source.journaltitleeLife
dc.source.volume10
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=5918&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/4884
dc.identifier.contextkey28282626
refterms.dateFOA2022-08-23T16:52:35Z
html.description.abstract<p>DNA loop extrusion by condensins and decatenation by DNA topoisomerase II (topo II) are thought to drive mitotic chromosome compaction and individualization. Here, we reveal that the linker histone H1.8 antagonizes condensins and topo II to shape mitotic chromosome organization. In vitro chromatin reconstitution experiments demonstrate that H1.8 inhibits binding of condensins and topo II to nucleosome arrays. Accordingly, H1.8 depletion in Xenopus egg extracts increased condensins and topo II levels on mitotic chromatin. Chromosome morphology and Hi-C analyses suggest that H1.8 depletion makes chromosomes thinner and longer through shortening the average loop size and reducing the DNA amount in each layer of mitotic loops. Furthermore, excess loading of condensins and topo II to chromosomes by H1.8 depletion causes hyper-chromosome individualization and dispersion. We propose that condensins and topo II are essential for chromosome individualization, but their functions are tuned by the linker histone to keep chromosomes together until anaphase.</p>
dc.identifier.submissionpathoapubs/4884
dc.contributor.departmentDepartment of Biochemistry and Molecular Pharmacology
dc.contributor.departmentProgram in Systems Biology
dc.source.pagese68918


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Copyright © 2021, Choppakatla et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Except where otherwise noted, this item's license is described as Copyright © 2021, Choppakatla et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.