Systematic analysis of essential yeast TAFs in genome-wide transcription and preinitiation complex assembly
Authors
Shen, Wu-ChengBhaumik, Sukesh R.
Causton, Helen C.
Simon, Itamar
Zhu, Xiaochun
Jennings, Ezra G.
Wang, Tseng-Hsing
Young, Richard A.
Green, Michael R.
UMass Chan Affiliations
Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular MedicineDocument Type
Journal ArticlePublication Date
2003-07-04Keywords
Base SequenceDNA Primers
*Genome, Fungal
Mutation
Promoter Regions (Genetics)
Saccharomyces cerevisiae
TATA-Binding Protein Associated Factors
Temperature
Transcription Factors
Life Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
The general transcription factor TFIID is composed of the TATA box binding protein (TBP) and a set of conserved TBP-associated factors (TAFs). Here we report the completion of genome-wide expression profiling analyses of yeast strains bearing temperature-sensitive mutations in each of the 13 essential TAFs. The percentage of the yeast genome dependent on each TAF ranges from 3% (TAF2) to 59-61% (TAF9). Approximately 84% of yeast genes are dependent upon one or more TAFs and 16% of yeast genes are TAF independent. In addition, this complete analysis defines three distinct classes of yeast promoters whose transcriptional requirements for TAFs differ substantially. Using this collection of temperature-sensitive mutants, we show that in all cases the transcriptional dependence for a TAF can be explained by a requirement for TBP recruitment and assembly of the preinitiation complex (PIC). Unexpectedly, these assembly experiments reveal that TAF11 and TAF13 appear to provide the critical functional contacts with TBP during PIC assembly. Collectively, our results confirm and extend the proposal that individual TAFs have selective transcriptional roles and distinct functions.Source
EMBO J. 2003 Jul 1;22(13):3395-402. Link to article on publisher's site
DOI
10.1093/emboj/cdg336Permanent Link to this Item
http://hdl.handle.net/20.500.14038/42118PubMed ID
12840001Related Resources
ae974a485f413a2113503eed53cd6c53
10.1093/emboj/cdg336