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    Developmental regulation of thyrotropin receptor gene expression in the fetal and neonatal rat thyroid: relation to thyroid morphology and to thyroid-specific gene expression

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    Authors
    Brown, Rosalind S.
    Shalhoub, Victoria
    Coulter, Sarah
    Alex, Sharon
    Joris, Isabelle
    DeVito, William J.
    Lian, Jane B.
    Stein, Gary S.
    UMass Chan Affiliations
    Department of Pathology
    Department of Cell Biology
    Department of Pediatrics
    Document Type
    Journal Article
    Publication Date
    1999-12-30
    Keywords
    Animals
    Animals, Newborn
    Blotting, Northern
    Female
    Gene Expression Regulation, Developmental
    In Situ Hybridization
    Iodide Peroxidase
    Pregnancy
    RNA, Messenger
    Rats
    Rats, Sprague-Dawley
    Receptors, Thyrotropin
    Thyroid Gland
    Up-Regulation
    Endocrinology, Diabetes, and Metabolism
    Life Sciences
    Medicine and Health Sciences
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    Link to Full Text
    http://dx.doi.org/10.1210/en.141.1.340
    Abstract
    The TSH receptor plays a pivotal role in thyroid gland function, growth, and differentiation, but little is known about its role or regulation in the fetus and neonate. To explore these questions, we systematically evaluated TSH receptor gene expression at the level of messenger RNA (mRNA) in thyroid glands obtained from rat fetuses and neonates, from 14 days gestation to day 5 of postnatal life. Results were compared with histological evidence of differentiation and to thyroid-specific gene expression. Northern blot and RT-PCR analysis revealed that TSH mRNA was first detected at low levels on fetal day 15, but it increased 3- to 15-fold on fetal days 17-18. Up-regulation of TSH receptor mRNA on fetal day 17-18 was accompanied by the first appearance of colloid formation and of follicular development on morphological examination. It was also paralleled by increased expression of the thyroid-specific genes thyroglobulin (Tg) and thyroid peroxidase. Unexpectedly, TSH mRNA abundance was 2- to 3-fold higher in pregnant dams than in nonpregnant adult females or adult males. In view of the 8-day lapse between the first appearance of the thyroid diverticulum and up-regulation of TSH receptor gene expression, we conclude that pituitary TSH, acting through its receptor, plays an important role in terminal thyroid maturation, but it is not involved earlier in gestation. Similarly, these data support previous evidence that the weak thyrotropic activity of human CG could not be of significance in early fetal thyroid gland development. The increased TSH receptor mRNA on fetal day 17-18 may be attributable to up-regulation by TSH, which is first secreted into the fetal circulation at this time. The significance of the increased TSH receptor expression during pregnancy remains to be explored.
    Source
    Endocrinology. 2000 Jan;141(1):340-5.
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/42142
    PubMed ID
    10614656
    Related Resources
    Link to Article in PubMed
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    UMass Chan Faculty and Researcher Publications

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