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    Thyroid hormone regulates the extracellular organization of laminin on astrocytes

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    Authors
    Farwell, Alan P.
    Dubord-Tomasetti, Susan A.
    UMass Chan Affiliations
    Molecular Endocrinology Laboratory
    Document Type
    Journal Article
    Publication Date
    1999-10-28
    Keywords
    Animals
    Animals, Newborn
    Astrocytes
    Cells, Cultured
    Electrophoresis, Polyacrylamide Gel
    Extracellular Matrix
    Immunohistochemistry
    Immunosorbent Techniques
    Laminin
    Mice
    Microscopy, Fluorescence
    Rats
    Sarcoma, Experimental
    Thyroid Hormones
    Thyroxine
    Triiodothyronine
    Life Sciences
    Medicine and Health Sciences
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    Link to Full Text
    https://doi.org/10.1210/en.140.11.5014
    Abstract
    Astrocytes produce laminin, a key extracellular matrix guidance molecule in the developing brain. Laminin is bound to transmembrane receptors on the surface of astrocytes known as integrins, which are, in turn, bound to the microfilament meshwork inside the astrocyte. Previous studies have shown that T4 regulates the pattern of integrin distribution in astrocytes by modulating the organization of the microfilaments. In this study, the effect of thyroid hormone on the secretion and topology of laminin in astrocytes was examined. Linear arrays of secreted laminin were observed on the surface of the T4-treated astrocytes within 10 h after seeding the cells onto poly-D-lysine-coated coverslips and became an organized meshwork by 24 h. In contrast, little if any laminin was identified on the surface of either hormone-deficient or T3-treated cells until 36 h after seeding and then was restricted to punctate deposits. Secretion of laminin into the medium by hormone-deficient and T3-treated cells was significantly greater than that by T4-treated cells. Conversely, deposition of laminin into the extracellular matrix was significantly greater in T4-treated cells than in hormone-deficient and T3-treated cells. Thyroid hormone had no effect on the production of laminin by astrocytes. These data show that T4 regulates the extracellular deposition and organization of laminin on the surface of astrocytes and provide a mechanism by which this morphogenic hormone can influence neuronal migration and axonal projection in the developing brain.
    Source

    Endocrinology. 1999 Nov;140(11):5014-21.

    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/42143
    PubMed ID
    10537126
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