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dc.contributor.authorOkulicz, William C.
dc.contributor.authorAce, Christopher I.
dc.contributor.authorLongcope, Christopher
dc.contributor.authorTast, Janet
dc.date2022-08-11T08:10:02.000
dc.date.accessioned2022-08-23T16:52:55Z
dc.date.available2022-08-23T16:52:55Z
dc.date.issued1996-11-01
dc.date.submitted2008-07-09
dc.identifier.citation<p>Endocrinology. 1996 Nov;137(11):4844-50.</p>
dc.identifier.issn0013-7227 (Print)
dc.identifier.pmid8895355
dc.identifier.urihttp://hdl.handle.net/20.500.14038/42154
dc.description.abstractThe ability to create artificial menstrual cycles in the rhesus monkey provides a model for studies on the regulation of genes and gene networks by estradiol or progesterone (P) in the primate endometrium. This model allowed us to create both a normal level of secretory phase P or an inadequate level of secretory phase P, i.e. endometria that cannot support implantation. The objective of our present study focused on PCR analyses of genes for several factors that are believed to be important in the proper maturation of the endometrium. Complementary DNA (cDNA) populations were prepared from endometria harvested on day 13 (peak E level), days 21-23 of an adequate secretory phase (PcDNA) and days 21-23 of an inadequate secretory phase (IcDNA). Although placental protein 14, leukemia inhibitory factor and 17-beta hydroxysteroid dehydrogenase displayed highly upregulated levels in PcDNA (P-activated genes), there was little or no up-regulation in IcDNA. Transforming growth factor-beta 2 and its receptor and insulin growth factor-I and its receptor were up-regulated in PcDNA, whereas little or no expression was observed in IcDNA. Regulators of the cell cycle and transcription, such as retinoblastoma, c-fos, and c-jun genes, were also greatly underexpressed in IcDNA compared with PcDNA. Interestingly, one gene that we studied, keratinocyte growth factor, that was up-regulated by P (peak E levels vs. PcDNA) was more highly expressed in IcDNA. This latter result suggests that low levels of circulating P are sufficient for expression of this gene, whereas high sustained P levels result in an autologous down-regulation. These data show that the regulation of genes that may play pivotal roles in endometrial maturation are differentially expressed in IcDNA vs. PcDNA and may, in part, characterize improper endometrial maturation.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=8895355&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.1210/en.137.11.4844
dc.subject17-Hydroxysteroid Dehydrogenases
dc.subjectAnimals
dc.subjectCell Cycle
dc.subjectDNA Primers
dc.subjectDNA, Complementary
dc.subjectEndometrium
dc.subjectFemale
dc.subject*Gene Expression Regulation
dc.subjectGenes, Retinoblastoma
dc.subjectGenes, fos
dc.subjectGenes, jun
dc.subjectGlycoproteins
dc.subjectGrowth Inhibitors
dc.subjectHumans
dc.subjectInsulin-Like Growth Factor I
dc.subject*Interleukin-6
dc.subjectLeukemia Inhibitory Factor
dc.subjectLymphokines
dc.subjectMacaca mulatta
dc.subject*Menstrual Cycle
dc.subjectPolymerase Chain Reaction
dc.subjectPregnancy Proteins
dc.subjectProgesterone
dc.subjectProtein-Serine-Threonine Kinases
dc.subjectProto-Oncogene Proteins c-fos
dc.subjectProto-Oncogene Proteins c-jun
dc.subjectReceptor, Epidermal Growth Factor
dc.subjectReceptor, IGF Type 1
dc.subjectReceptors, Transforming Growth Factor beta
dc.subjectRetinoblastoma Protein
dc.subjectTranscription, Genetic
dc.subjectTransforming Growth Factor beta
dc.subjectUp-Regulation
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleAnalysis of differential gene regulation in adequate versus inadequate secretory-phase endometrial complementary deoxyribonucleic acid populations from the rhesus monkey
dc.typeJournal Article
dc.source.journaltitleEndocrinology
dc.source.volume137
dc.source.issue11
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/527
dc.identifier.contextkey545009
html.description.abstract<p>The ability to create artificial menstrual cycles in the rhesus monkey provides a model for studies on the regulation of genes and gene networks by estradiol or progesterone (P) in the primate endometrium. This model allowed us to create both a normal level of secretory phase P or an inadequate level of secretory phase P, i.e. endometria that cannot support implantation. The objective of our present study focused on PCR analyses of genes for several factors that are believed to be important in the proper maturation of the endometrium. Complementary DNA (cDNA) populations were prepared from endometria harvested on day 13 (peak E level), days 21-23 of an adequate secretory phase (PcDNA) and days 21-23 of an inadequate secretory phase (IcDNA). Although placental protein 14, leukemia inhibitory factor and 17-beta hydroxysteroid dehydrogenase displayed highly upregulated levels in PcDNA (P-activated genes), there was little or no up-regulation in IcDNA. Transforming growth factor-beta 2 and its receptor and insulin growth factor-I and its receptor were up-regulated in PcDNA, whereas little or no expression was observed in IcDNA. Regulators of the cell cycle and transcription, such as retinoblastoma, c-fos, and c-jun genes, were also greatly underexpressed in IcDNA compared with PcDNA. Interestingly, one gene that we studied, keratinocyte growth factor, that was up-regulated by P (peak E levels vs. PcDNA) was more highly expressed in IcDNA. This latter result suggests that low levels of circulating P are sufficient for expression of this gene, whereas high sustained P levels result in an autologous down-regulation. These data show that the regulation of genes that may play pivotal roles in endometrial maturation are differentially expressed in IcDNA vs. PcDNA and may, in part, characterize improper endometrial maturation.</p>
dc.identifier.submissionpathoapubs/527
dc.contributor.departmentDepartment of Obstetrics/Gynecology
dc.source.pages4844-50


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