Second-site reversion of a structural defect in bacteriophage T4 lysozyme
UMass Chan AffiliationsDepartment of Molecular Genetics and Microbiology
Document TypeJournal Article
Molecular Sequence Data
Polymerase Chain Reaction
Sequence Analysis, DNA
Medicine and Health Sciences
MetadataShow full item record
AbstractA plasmid-borne gene encoding bacteriophage T4 lysozyme with a structural mutation, Tyr161-Ala, was mutagenized by by the use of polymerase chain reaction. The mutagenized gene was inserted into a specialized bacteriophage lambda cloning vector that must acquire a functional lysozyme gene in order to form plaques. Functional variants of the mutant lysozyme were selected. Three compensatory second-site revertants were obtained: Thr152-Met, Lys43-Ile, and Thr151-Ala. The effects of these mutations are interpreted in light of previous structural and genetic studies of T4 lysozyme.
FASEB J. 1996 Jan;10(1):159-63.
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/42199