Show simple item record

dc.contributor.authorBaker, Richard E.
dc.contributor.authorRogers, Kelly
dc.date2022-08-11T08:10:03.000
dc.date.accessioned2022-08-23T16:53:17Z
dc.date.available2022-08-23T16:53:17Z
dc.date.issued2005-08-05
dc.date.submitted2008-07-15
dc.identifier.citation<p>Genetics. 2005 Dec;171(4):1463-75. Epub 2005 Aug 3. <a href="http://dx.doi.org/10.1534/genetics.105.046458">Link to article on publisher's site</a></p>
dc.identifier.issn0016-6731 (Print)
dc.identifier.doi10.1534/genetics.105.046458
dc.identifier.pmid16079225
dc.identifier.urihttp://hdl.handle.net/20.500.14038/42238
dc.description.abstractCentromere DNA element II (CDEII) of budding yeast centromeres is an AT-rich sequence essential for centromere (CEN) function. Sequence analysis of Saccharomyces cerevisiae CDEIIs revealed that A(5-7)/T(5-7) tracts are statistically overrepresented at the expense of AA/TT and alternating AT. To test the hypothesis that this nonrandom sequence organization is functionally important, a CEN library in which the CDEII sequences were randomized was generated. The library was screened for functional and nonfunctional members following centromere replacement in vivo. Functional CENs contained CDEIIs with the highly biased A(n)/T(n) run distribution of native centromeres, while nonfunctional CDEIIs resembled those picked from the library at random. Run content, defined as the fraction of residues present in runs of four or more nucleotides, of the functional and nonfunctional CDEII populations differed significantly (P < 0.001). Computer searches of the genome for regions with an A + T content comparable to CDEIIs revealed that such loci are not unique to centromeres, but for 14 of the 16 chromosomes the AT-rich locus with the highest A(n > or =4) + T(n > or =4) run content was the centromere. Thus, the distinctive and nonrandom sequence organization of CDEII is important for centromere function and possesses informational content that could contribute to the determination of centromere identity.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=16079225&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1350974/
dc.subjectAT Rich Sequence
dc.subjectBase Composition
dc.subjectBase Sequence
dc.subjectCentromere
dc.subjectChromosomes, Fungal
dc.subjectComputational Biology
dc.subjectDNA Primers
dc.subjectDNA, Fungal
dc.subjectGene Library
dc.subjectGenomics
dc.subjectMolecular Sequence Data
dc.subjectSaccharomyces cerevisiae
dc.subjectSequence Analysis, DNA
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleGenetic and genomic analysis of the AT-rich centromere DNA element II of Saccharomyces cerevisiae
dc.typeJournal Article
dc.source.journaltitleGenetics
dc.source.volume171
dc.source.issue4
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/603
dc.identifier.contextkey549032
html.description.abstract<p>Centromere DNA element II (CDEII) of budding yeast centromeres is an AT-rich sequence essential for centromere (CEN) function. Sequence analysis of Saccharomyces cerevisiae CDEIIs revealed that A(5-7)/T(5-7) tracts are statistically overrepresented at the expense of AA/TT and alternating AT. To test the hypothesis that this nonrandom sequence organization is functionally important, a CEN library in which the CDEII sequences were randomized was generated. The library was screened for functional and nonfunctional members following centromere replacement in vivo. Functional CENs contained CDEIIs with the highly biased A(n)/T(n) run distribution of native centromeres, while nonfunctional CDEIIs resembled those picked from the library at random. Run content, defined as the fraction of residues present in runs of four or more nucleotides, of the functional and nonfunctional CDEII populations differed significantly (P < 0.001). Computer searches of the genome for regions with an A + T content comparable to CDEIIs revealed that such loci are not unique to centromeres, but for 14 of the 16 chromosomes the AT-rich locus with the highest A(n > or =4) + T(n > or =4) run content was the centromere. Thus, the distinctive and nonrandom sequence organization of CDEII is important for centromere function and possesses informational content that could contribute to the determination of centromere identity.</p>
dc.identifier.submissionpathoapubs/603
dc.contributor.departmentDepartment of Molecular Genetics and Microbiology
dc.source.pages1463-75


This item appears in the following Collection(s)

Show simple item record