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dc.contributor.authorLian, Jane B.
dc.contributor.authorStein, Gary S.
dc.date2022-08-11T08:10:03.000
dc.date.accessioned2022-08-23T16:53:27Z
dc.date.available2022-08-23T16:53:27Z
dc.date.issued1995-01-01
dc.date.submitted2008-07-15
dc.identifier.citationIowa Orthop J. 1995;15:118-40.
dc.identifier.issn1541-5457 (Print)
dc.identifier.pmid7634023
dc.identifier.urihttp://hdl.handle.net/20.500.14038/42277
dc.description.abstractThe formation of bone tissue involves multiple activities of the osteoblast. The combined application of molecular, biochemical, histochemical and ultrastructural approaches has defined stages in the development of the osteoblast phenotype with each subpopulation of cells exhibiting unique morphologic and functional properties in relation to the ordered deposition of the mineralized bone extracellular matrix (ECM). Peak levels of expressed genes reflect a maturational sequence of osteoblast growth and differentiation characterized by three principal periods: proliferation, ECM maturation and mineralization. A plethora of new information in the past several years provides the basis for insight into molecular mechanisms regulating the development and activities of differentiating osteoblasts. These new concepts will be discussed within the context of understanding cellular responses of bone tissue. To be considered are the following: 1) maturational stages of the osteoblast reflected by the selective expression of transcription factors (e.g., oncogenes, cyclins, homeodomain proteins) and phenotypic genes that provide signals for differentiation through the osteoblast lineage; 2) role of the extracellular matrix in mediating osteoblast growth and differentiation; 3) osteoblast stage specific responses to physiologic mediators (e.g., growth factors and hormones); 4) the developmentally regulated expression and selective responses of osteoblast phenotypic genes are supported by cooperative, synergistic and/or antagonistic activities at multiple basal and enhancer or suppressor sequences in gene promoters; and 5) deregulation of these control mechanisms in transformed osteoblasts and osteosarcoma cells.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=7634023&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2329080/pdf/iowaorthj00019-0143.pdf
dc.subjectAnimals
dc.subjectCell Differentiation
dc.subjectCell Division
dc.subjectCell Line
dc.subjectExtracellular Matrix
dc.subjectGene Expression
dc.subjectGene Expression Regulation, Neoplastic
dc.subjectHistones
dc.subjectHumans
dc.subjectOsteoblasts
dc.subjectOsteocalcin
dc.subjectOsteosarcoma
dc.subjectPhenotype
dc.subjectPromoter Regions (Genetics)
dc.subjectTransforming Growth Factor beta
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleDevelopment of the osteoblast phenotype: molecular mechanisms mediating osteoblast growth and differentiation
dc.typeArticle
dc.source.journaltitleThe Iowa orthopaedic journal
dc.source.volume15
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/639
dc.identifier.contextkey549068
html.description.abstract<p>The formation of bone tissue involves multiple activities of the osteoblast. The combined application of molecular, biochemical, histochemical and ultrastructural approaches has defined stages in the development of the osteoblast phenotype with each subpopulation of cells exhibiting unique morphologic and functional properties in relation to the ordered deposition of the mineralized bone extracellular matrix (ECM). Peak levels of expressed genes reflect a maturational sequence of osteoblast growth and differentiation characterized by three principal periods: proliferation, ECM maturation and mineralization. A plethora of new information in the past several years provides the basis for insight into molecular mechanisms regulating the development and activities of differentiating osteoblasts. These new concepts will be discussed within the context of understanding cellular responses of bone tissue. To be considered are the following: 1) maturational stages of the osteoblast reflected by the selective expression of transcription factors (e.g., oncogenes, cyclins, homeodomain proteins) and phenotypic genes that provide signals for differentiation through the osteoblast lineage; 2) role of the extracellular matrix in mediating osteoblast growth and differentiation; 3) osteoblast stage specific responses to physiologic mediators (e.g., growth factors and hormones); 4) the developmentally regulated expression and selective responses of osteoblast phenotypic genes are supported by cooperative, synergistic and/or antagonistic activities at multiple basal and enhancer or suppressor sequences in gene promoters; and 5) deregulation of these control mechanisms in transformed osteoblasts and osteosarcoma cells.</p>
dc.identifier.submissionpathoapubs/639
dc.contributor.departmentDepartment of Cell Biology
dc.source.pages118-40


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