ATP-dependent and ATP-independent roles for the Rad54 chromatin remodeling enzyme during recombinational repair of a DNA double strand break
UMass Chan Affiliations
Program in Molecular MedicineDocument Type
Journal ArticlePublication Date
2005-01-18Keywords
Adenosine TriphosphatasesAdenosine Triphosphate
Blotting, Southern
Chromatin
DNA
*DNA Damage
DNA Repair
Fenfluramine
Fungal Proteins
Genome
Humans
Hydrolysis
Immunoprecipitation
Micrococcal Nuclease
Models, Genetic
Plasmids
*Recombination, Genetic
Reverse Transcriptase Polymerase Chain Reaction
Saccharomyces cerevisiae Proteins
Time Factors
Life Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
The efficient and accurate repair of DNA double strand breaks (DSBs) is critical to cell survival, and defects in this process can lead to genome instability and cancers. In eukaryotes, the Rad52 group of proteins dictates the repair of DSBs by the error-free process of homologous recombination (HR). A critical step in eukaryotic HR is the formation of the initial Rad51-single-stranded DNA presynaptic nucleoprotein filament. This presynaptic filament participates in a homology search process that leads to the formation of a DNA joint molecule and recombinational repair of the DSB. Recently, we showed that the Rad54 protein functions as a mediator of Rad51 binding to single-stranded DNA, and here, we find that this activity does not require ATP hydrolysis. We also identify a novel Rad54-dependent chromatin remodeling event that occurs in vivo during the DNA strand invasion step of HR. This ATP-dependent remodeling activity of Rad54 appears to control subsequent steps in the HR process.Source
J Biol Chem. 2005 Mar 18;280(11):10855-60. Epub 2005 Jan 14. Link to article on publisher's siteDOI
10.1074/jbc.M414388200Permanent Link to this Item
http://hdl.handle.net/20.500.14038/42338PubMed ID
15653683Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1074/jbc.M414388200