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dc.contributor.authorRoy, Kanaklata
dc.contributor.authorde la Serna, Ivana L.
dc.contributor.authorImbalzano, Anthony N.
dc.date2022-08-11T08:10:03.000
dc.date.accessioned2022-08-23T16:53:52Z
dc.date.available2022-08-23T16:53:52Z
dc.date.issued2002-07-10
dc.date.submitted2008-08-04
dc.identifier.citationJ Biol Chem. 2002 Sep 13;277(37):33818-24. Epub 2002 Jul 8. <a href="http://dx.doi.org/10.1074/jbc.M205159200">Link to article on publisher's site</a>
dc.identifier.issn0021-9258 (Print)
dc.identifier.doi10.1074/jbc.M205159200
dc.identifier.pmid12105204
dc.identifier.urihttp://hdl.handle.net/20.500.14038/42373
dc.description.abstractThe myogenic basic helix-loop-helix family of transcription factors, MyoD, Myf5, myogenin, and MRF4, can each activate the muscle differentiation program when ectopically expressed in non-muscle cells. SWI/SNF complexes are ATP-dependent chromatin remodeling enzymes. We demonstrated previously that SWI/SNF enzymes promote MyoD-mediated muscle differentiation. To ascertain the requirement for SWI/SNF enzymes in muscle differentiation mediated by different MyoD family members, we examined MyoD, Myf5, MRF4, and myogenin-mediated induction of muscle differentiation in cells expressing dominant negative versions of BRG1 or BRM-based SWI/SNF enzymes. We demonstrated that expression of dominant negative BRG1 or BRM inhibited the induction of muscle-specific gene expression by Myf5 and MRF4; however, myogenin failed to induce measurable quantities of muscle-specific mRNAs, even in cells not expressing dominant negative SWI/SNF. In contrast, all four myogenic regulators induced expression of the cell cycle regulators p21, Rb, and cyclin D3 and promoted cell cycle arrest independently of the SWI/SNF enzymes. We proposed that SWI/SNF enzymes are required for the induction of all muscle-specific gene expression by MyoD, Myf5, and MRF4, whereas induction of the cell cycle regulators, p21, Rb, and cyclin D3 occurred independently of SWI/SNF function.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=12105204&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1074/jbc.M205159200
dc.subject3T3 Cells
dc.subjectAnimals
dc.subjectCell Cycle
dc.subjectCell Differentiation
dc.subjectChromatin
dc.subjectChromosomal Proteins, Non-Histone
dc.subjectCyclins
dc.subject*DNA-Binding Proteins
dc.subjectGene Expression Regulation, Developmental
dc.subjectHelix-Loop-Helix Motifs
dc.subjectHumans
dc.subjectMice
dc.subjectMuscle Proteins
dc.subjectMuscle, Skeletal
dc.subjectMyoD Protein
dc.subjectMyogenic Regulatory Factor 5
dc.subjectMyogenic Regulatory Factors
dc.subjectMyogenin
dc.subject*Trans-Activators
dc.subjectTranscription Factors
dc.subjectCell Biology
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleThe myogenic basic helix-loop-helix family of transcription factors shows similar requirements for SWI/SNF chromatin remodeling enzymes during muscle differentiation in culture
dc.typeJournal Article
dc.source.journaltitleThe Journal of biological chemistry
dc.source.volume277
dc.source.issue37
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/728
dc.identifier.contextkey564643
html.description.abstract<p>The myogenic basic helix-loop-helix family of transcription factors, MyoD, Myf5, myogenin, and MRF4, can each activate the muscle differentiation program when ectopically expressed in non-muscle cells. SWI/SNF complexes are ATP-dependent chromatin remodeling enzymes. We demonstrated previously that SWI/SNF enzymes promote MyoD-mediated muscle differentiation. To ascertain the requirement for SWI/SNF enzymes in muscle differentiation mediated by different MyoD family members, we examined MyoD, Myf5, MRF4, and myogenin-mediated induction of muscle differentiation in cells expressing dominant negative versions of BRG1 or BRM-based SWI/SNF enzymes. We demonstrated that expression of dominant negative BRG1 or BRM inhibited the induction of muscle-specific gene expression by Myf5 and MRF4; however, myogenin failed to induce measurable quantities of muscle-specific mRNAs, even in cells not expressing dominant negative SWI/SNF. In contrast, all four myogenic regulators induced expression of the cell cycle regulators p21, Rb, and cyclin D3 and promoted cell cycle arrest independently of the SWI/SNF enzymes. We proposed that SWI/SNF enzymes are required for the induction of all muscle-specific gene expression by MyoD, Myf5, and MRF4, whereas induction of the cell cycle regulators, p21, Rb, and cyclin D3 occurred independently of SWI/SNF function.</p>
dc.identifier.submissionpathoapubs/728
dc.contributor.departmentDepartment of Cell Biology
dc.source.pages33818-24


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