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dc.contributor.authorLi, Hui
dc.contributor.authorChen, J. Don
dc.date2022-08-11T08:10:04.000
dc.date.accessioned2022-08-23T16:54:08Z
dc.date.available2022-08-23T16:54:08Z
dc.date.issued1998-03-06
dc.date.submitted2008-08-04
dc.identifier.citation<p>This research was originally published in: J Biol Chem. 1998 Mar 6;273(10):5948-54. <a href="https://doi.org/10.1074/jbc.273.10.5948" target="_blank" title="Link to article on publisher's site">Link to article on publisher's site</a></p>
dc.identifier.issn0021-9258 (Print)
dc.identifier.doi10.1074/jbc.273.10.5948
dc.identifier.pmid9488734
dc.identifier.urihttp://hdl.handle.net/20.500.14038/42431
dc.description.abstractTranscriptional coactivators are involved in gene activation by nuclear hormone receptors. The receptor-associated coactivator 3 (RAC3) was recently identified to be highly related to the steroid receptor coactivator-1 and transcriptional intermediate factor 2, thereby establishing a novel family of nuclear receptor coactivators. In this study, we identified a RAC3 fragment containing three LXXLL motifs conserved among this family, which is sufficient to mediate nuclear receptor interaction in vivo and in vitro. Point mutations that disrupt ligand-dependent activation function of the receptor inhibited the interaction. We found that a 162-amino acid fragment of RAC3 conferred transcriptional activation and recruited the CREB-binding protein and that three distinct LXXLL motifs mediated the transcriptional activation. A trimeric far Western analysis demonstrated the formation of a ternary complex containing CREB-binding protein, RAC3, and the receptor. In addition, we showed that RAC3, transcriptional intermediate factor 2, and steroid receptor coactivator-1 are expressed in specific tissues and cancer cells and that RAC3 transcript is directly up-regulated by retinoid treatment. These results suggest that RAC3 may contribute to amplified transcriptional responses through both recruitment of additional coactivators and autoregulation by the receptor-coactivator complex.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=9488734&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.1074/jbc.273.10.5948
dc.rights© 1998 by The American Society for Biochemistry and Molecular Biology, Inc. Publisher PDF posted after 12 months as allowed by the publisher's author rights policy at https://www.asbmb.org/journals-news/editorial-policies.
dc.subjectAnimals
dc.subjectCREB-Binding Protein
dc.subjectCell Line
dc.subjectCercopithecus aethiops
dc.subjectHistone Acetyltransferases
dc.subjectNuclear Proteins
dc.subjectPeptide Fragments
dc.subjectRNA, Messenger
dc.subjectReceptors, Steroid
dc.subjectRetinoids
dc.subjectTrans-Activation (Genetics)
dc.subjectTrans-Activators
dc.subjectTranscription Factors
dc.subjectTransfection
dc.subjecttranscriptional coactivators
dc.subjectnuclear hormone receptors
dc.subjectreceptor-associated coactivator 3
dc.subjectRAC3
dc.subjectnuclear receptor coactivators
dc.subjectBiochemistry
dc.subjectCell Biology
dc.subjectCellular and Molecular Physiology
dc.subjectMolecular Biology
dc.titleThe receptor-associated coactivator 3 activates transcription through CREB-binding protein recruitment and autoregulation
dc.typeJournal Article
dc.source.journaltitleThe Journal of biological chemistry
dc.source.volume273
dc.source.issue10
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1780&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/781
dc.identifier.contextkey564696
refterms.dateFOA2022-08-23T16:54:08Z
html.description.abstract<p>Transcriptional coactivators are involved in gene activation by nuclear hormone receptors. The receptor-associated coactivator 3 (RAC3) was recently identified to be highly related to the steroid receptor coactivator-1 and transcriptional intermediate factor 2, thereby establishing a novel family of nuclear receptor coactivators. In this study, we identified a RAC3 fragment containing three LXXLL motifs conserved among this family, which is sufficient to mediate nuclear receptor interaction in vivo and in vitro. Point mutations that disrupt ligand-dependent activation function of the receptor inhibited the interaction. We found that a 162-amino acid fragment of RAC3 conferred transcriptional activation and recruited the CREB-binding protein and that three distinct LXXLL motifs mediated the transcriptional activation. A trimeric far Western analysis demonstrated the formation of a ternary complex containing CREB-binding protein, RAC3, and the receptor. In addition, we showed that RAC3, transcriptional intermediate factor 2, and steroid receptor coactivator-1 are expressed in specific tissues and cancer cells and that RAC3 transcript is directly up-regulated by retinoid treatment. These results suggest that RAC3 may contribute to amplified transcriptional responses through both recruitment of additional coactivators and autoregulation by the receptor-coactivator complex.</p>
dc.identifier.submissionpathoapubs/781
dc.contributor.departmentDepartment of Pharmacology and Molecular Toxicology
dc.source.pages5948-54


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