Regulation of cell adhesion receptors by transforming growth factor-beta. Regulation of vitronectin receptor and LFA-1
dc.contributor.author | Ignotz, Ronald A. | |
dc.contributor.author | Heino, Jyrki | |
dc.contributor.author | Massague, Joan | |
dc.date | 2022-08-11T08:10:05.000 | |
dc.date.accessioned | 2022-08-23T16:54:38Z | |
dc.date.available | 2022-08-23T16:54:38Z | |
dc.date.issued | 1989-01-05 | |
dc.date.submitted | 2008-08-15 | |
dc.identifier.citation | J Biol Chem. 1989 Jan 5;264(1):389-92. | |
dc.identifier.issn | 0021-9258 (Print) | |
dc.identifier.pmid | 2462560 | |
dc.identifier.uri | http://hdl.handle.net/20.500.14038/42546 | |
dc.description.abstract | We have examined the ability of transforming growth factor-beta 1 (TGF-beta 1) to regulate the expression of members of the alpha beta 2 and alpha beta 3 families of integrins. TGF-beta 1 elevates the expression of vitronectin receptors (alpha v beta 3 integrin) in all cells examined including WI-38 human lung fibroblasts, 3T3-L1 mouse fibroblasts, and MG-63 human osteogenic sarcoma cells. TGF-beta 1 action increases the level of mRNA and the synthesis of vitronectin receptor subunits with t1/2 o 3-4 h and 6 h, respectively. TGF-beta 1 up-regulates expression of the intercellular adhesion receptor, LFA-1 (alpha L beta 2), in THP-1 human monocytic leukemia cells by increasing the synthesis of alpha L subunit but not beta 2 subunit. The increase in alpha L synthesis and assembly into LFA-1 complexes induced by TGF-beta 1 occurs in parallel with elevated fibronectin receptor synthesis in THP-1 cells. These responses to TGF-beta 1 are lost upon phorbol ester-induced differentiation of THP-1 cells into the macrophage phenotype. The results suggest a role of TGF-beta in the regulation of cell-matrix interactions mediated by vitronectin receptors and cell-cell interactions mediated by LFA-1 in the immune system. | |
dc.language.iso | en_US | |
dc.relation | <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=2462560&dopt=Abstract">Link to Article in PubMed</a> | |
dc.relation.url | http://www.jbc.org/content/264/1/389.full.pdf+html | |
dc.subject | Animals | |
dc.subject | Antigens, Differentiation | |
dc.subject | Antigens, Surface | |
dc.subject | *Cell Adhesion | |
dc.subject | Cell Adhesion Molecules | |
dc.subject | Cell Line | |
dc.subject | Humans | |
dc.subject | Lymphocyte Function-Associated Antigen-1 | |
dc.subject | Macromolecular Substances | |
dc.subject | Membrane Glycoproteins | |
dc.subject | Mice | |
dc.subject | RNA, Messenger | |
dc.subject | Receptors, Immunologic | |
dc.subject | Receptors, Vitronectin | |
dc.subject | Transforming Growth Factors | |
dc.subject | Life Sciences | |
dc.subject | Medicine and Health Sciences | |
dc.title | Regulation of cell adhesion receptors by transforming growth factor-beta. Regulation of vitronectin receptor and LFA-1 | |
dc.type | Journal Article | |
dc.source.journaltitle | The Journal of biological chemistry | |
dc.source.volume | 264 | |
dc.source.issue | 1 | |
dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/oapubs/885 | |
dc.identifier.contextkey | 579774 | |
html.description.abstract | <p>We have examined the ability of transforming growth factor-beta 1 (TGF-beta 1) to regulate the expression of members of the alpha beta 2 and alpha beta 3 families of integrins. TGF-beta 1 elevates the expression of vitronectin receptors (alpha v beta 3 integrin) in all cells examined including WI-38 human lung fibroblasts, 3T3-L1 mouse fibroblasts, and MG-63 human osteogenic sarcoma cells. TGF-beta 1 action increases the level of mRNA and the synthesis of vitronectin receptor subunits with t1/2 o 3-4 h and 6 h, respectively. TGF-beta 1 up-regulates expression of the intercellular adhesion receptor, LFA-1 (alpha L beta 2), in THP-1 human monocytic leukemia cells by increasing the synthesis of alpha L subunit but not beta 2 subunit. The increase in alpha L synthesis and assembly into LFA-1 complexes induced by TGF-beta 1 occurs in parallel with elevated fibronectin receptor synthesis in THP-1 cells. These responses to TGF-beta 1 are lost upon phorbol ester-induced differentiation of THP-1 cells into the macrophage phenotype. The results suggest a role of TGF-beta in the regulation of cell-matrix interactions mediated by vitronectin receptors and cell-cell interactions mediated by LFA-1 in the immune system.</p> | |
dc.identifier.submissionpath | oapubs/885 | |
dc.contributor.department | Department of Surgery | |
dc.contributor.department | Department of Biochemistry | |
dc.source.pages | 389-92 |