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dc.contributor.authorWaite, Debra J.
dc.contributor.authorAppel, Michael C.
dc.contributor.authorHandler, Eugene S.
dc.contributor.authorMordes, John P.
dc.contributor.authorRossini, Aldo A.
dc.contributor.authorGreiner, Dale L.
dc.date2022-08-11T08:10:05.000
dc.date.accessioned2022-08-23T16:54:45Z
dc.date.available2022-08-23T16:54:45Z
dc.date.issued1996-06-01
dc.date.submitted2007-12-10
dc.identifier.citationAm J Pathol. 1996 Jun;148(6):2043-56.
dc.identifier.issn0002-9440 (Print)
dc.identifier.pmid8669488
dc.identifier.urihttp://hdl.handle.net/20.500.14038/42574
dc.description.abstractRT6 is a cell surface alloantigen that identifies a regulatory subset of peripheral T cells in the rat. Diabetes-prone BB rats are deficient in peripheral RT6+ T cells and develop spontaneous autoimmune insulin-dependent diabetes mellitus. Diabetes-resistant BB rats have normal numbers of RT6+ T cells, and insulin-dependent diabetes mellitus can be induced in these animals by in vivo depletion of peripheral RT6+ cells. Athymic rats are also severely deficient in peripheral RT6+ T cells. Although very different with respect to the peripheral RT6+ cell compartment, normal, athymic, and diabetes-prone BB rats all generate RT6+ intestinal epithelial lymphocytes (IELs). The goal of these studies was to analyze the ontogeny of RT6+ IELs and peripheral lymphoid cells by in situ immunohistochemistry. We observed the following. 1) RT6+ IELs appear before alpha(beta) T-cell-receptor- expressing IELs in diabetes-prone BB, diabetes-resistant BB, and athymic WAG rats. 2) In vivo depletion of peripheral RT6+ T cells in diabetes-resistant BB rats using a cytotoxic monoclonal antibody is not accompanied by depletion of RT6+ IELs. 3) A population of RT6+ T-cell-receptor-negative IELs is present in normal, euthymic diabetes-resistant BB rats, constitutes a larger percentage of the euthymic but lymphopenic diabetes-prone BB rat IEL population, and is the predominant IEL phenotype in athymic WAG rats. These results suggest that RT6+ cells are composed of both thymus-dependent and thymus-independent cell subsets that have different developmental characteristics and may differ in function.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=8669488&dopt=Abstract ">Link to article in PubMed</a>
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC1861642/?tool=pubmed
dc.subject*ADP Ribose Transferases
dc.subjectAnimals
dc.subjectAnimals, Newborn
dc.subjectAntigens, Differentiation, T-Lymphocyte
dc.subjectBlotting, Western
dc.subjectFemale
dc.subjectFlow Cytometry
dc.subjectHistocompatibility Antigens
dc.subjectImmunoblotting
dc.subjectImmunohistochemistry
dc.subjectIntestinal Mucosa
dc.subjectIsoantibodies
dc.subjectLymphoid Tissue
dc.subjectMale
dc.subject*Membrane Glycoproteins
dc.subjectPhenotype
dc.subjectRats
dc.subjectRats, Inbred BB
dc.subjectRats, Nude
dc.subjectReceptors, Antigen, T-Cell, alpha-beta
dc.subjectSpleen
dc.subjectT-Lymphocyte Subsets
dc.subjectThymus Gland
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleOntogeny and immunohistochemical localization of thymus-dependent and thymus-independent RT6+ cells in the rat
dc.typeJournal Article
dc.source.journaltitleThe American journal of pathology
dc.source.volume148
dc.source.issue6
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/91
dc.identifier.contextkey403150
html.description.abstract<p>RT6 is a cell surface alloantigen that identifies a regulatory subset of peripheral T cells in the rat. Diabetes-prone BB rats are deficient in peripheral RT6+ T cells and develop spontaneous autoimmune insulin-dependent diabetes mellitus. Diabetes-resistant BB rats have normal numbers of RT6+ T cells, and insulin-dependent diabetes mellitus can be induced in these animals by in vivo depletion of peripheral RT6+ cells. Athymic rats are also severely deficient in peripheral RT6+ T cells. Although very different with respect to the peripheral RT6+ cell compartment, normal, athymic, and diabetes-prone BB rats all generate RT6+ intestinal epithelial lymphocytes (IELs). The goal of these studies was to analyze the ontogeny of RT6+ IELs and peripheral lymphoid cells by in situ immunohistochemistry. We observed the following. 1) RT6+ IELs appear before alpha(beta) T-cell-receptor- expressing IELs in diabetes-prone BB, diabetes-resistant BB, and athymic WAG rats. 2) In vivo depletion of peripheral RT6+ T cells in diabetes-resistant BB rats using a cytotoxic monoclonal antibody is not accompanied by depletion of RT6+ IELs. 3) A population of RT6+ T-cell-receptor-negative IELs is present in normal, euthymic diabetes-resistant BB rats, constitutes a larger percentage of the euthymic but lymphopenic diabetes-prone BB rat IEL population, and is the predominant IEL phenotype in athymic WAG rats. These results suggest that RT6+ cells are composed of both thymus-dependent and thymus-independent cell subsets that have different developmental characteristics and may differ in function.</p>
dc.identifier.submissionpathoapubs/91
dc.contributor.departmentDepartment of Medicine, Diabetes Division
dc.source.pages2043-56


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