UMass Chan Affiliations
Proteomic Mass Spectrometry LaboratoryDepartment of Cell Biology
Program in Molecular Medicine
Document Type
Journal ArticlePublication Date
2005-07-07Keywords
AnimalsChlamydomonas reinhardtii
Cilia
Conserved Sequence
Eukaryotic Cells
Evolution, Molecular
Mass Spectrometry
Microscopy, Electron, Transmission
Molecular Motor Proteins
Proteome
Proteomics
Protozoan Proteins
Signal Transduction
Cell Biology
Laboratory and Basic Science Research
Metadata
Show full item recordAbstract
Cilia and flagella are widespread cell organelles that have been highly conserved throughout evolution and play important roles in motility, sensory perception, and the life cycles of eukaryotes ranging from protists to humans. Despite the ubiquity and importance of these organelles, their composition is not well known. Here we use mass spectrometry to identify proteins in purified flagella from the green alga Chlamydomonas reinhardtii. 360 proteins were identified with high confidence, and 292 more with moderate confidence. 97 out of 101 previously known flagellar proteins were found, indicating that this is a very complete dataset. The flagellar proteome is rich in motor and signal transduction components, and contains numerous proteins with homologues associated with diseases such as cystic kidney disease, male sterility, and hydrocephalus in humans and model vertebrates. The flagellum also contains many proteins that are conserved in humans but have not been previously characterized in any organism. The results indicate that flagella are far more complex than previously estimated.Source
J Cell Biol. 2005 Jul 4;170(1):103-13. Link to article on publisher's siteDOI
10.1083/jcb.200504008Permanent Link to this Item
http://hdl.handle.net/20.500.14038/42577PubMed ID
15998802Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1083/jcb.200504008