Processing of endogenous pre-mRNAs in association with SC-35 domains is gene specific
UMass Chan Affiliations
Department of Cell BiologyDocument Type
Journal ArticlePublication Date
1999-02-26Keywords
Cell LineDystrophin
Humans
In Situ Hybridization, Fluorescence
Myosin Heavy Chains
Nuclear Proteins
RNA Precursors
RNA Processing, Post-Transcriptional
RNA Splicing
RNA, Messenger
*Ribonucleoproteins
Spliceosomes
Transcription, Genetic
Cell Biology
Life Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
Analysis of six endogenous pre-mRNAs demonstrates that localization at the periphery or within splicing factor-rich (SC-35) domains is not restricted to a few unusually abundant pre-mRNAs, but is apparently a more common paradigm of many protein-coding genes. Different genes are preferentially transcribed and their RNAs processed in different compartments relative to SC-35 domains. These differences do not simply correlate with the complexity, nuclear abundance, or position within overall nuclear space. The distribution of spliceosome assembly factor SC-35 did not simply mirror the distribution of individual pre-mRNAs, but rather suggested that individual domains contain both specific pre-mRNA(s) as well as excess splicing factors. This is consistent with a multifunctional compartment, to which some gene loci and their RNAs have access and others do not. Despite similar molar abundance in muscle fiber nuclei, nascent transcript "trees" of highly complex dystrophin RNA are cotranscriptionally spliced outside of SC-35 domains, whereas posttranscriptional "tracks" of more mature myosin heavy chain transcripts overlap domains. Further analyses supported that endogenous pre-mRNAs exhibit distinct structural organization that may reflect not only the expression and complexity of the gene, but also constraints of its chromosomal context and kinetics of its RNA metabolism.Source
J Cell Biol. 1999 Feb 22;144(4):617-29. Link to article on publisher's websiteDOI
10.1083/jcb.144.4.617Permanent Link to this Item
http://hdl.handle.net/20.500.14038/42601PubMed ID
10037785Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1083/jcb.144.4.617