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dc.contributor.authorSmith, Kelly P.
dc.contributor.authorMoen, Phillip T. Jr.
dc.contributor.authorWydner, Karen L.
dc.contributor.authorColeman, John R.
dc.contributor.authorLawrence, Jeanne B.
dc.date2022-08-11T08:10:05.000
dc.date.accessioned2022-08-23T16:54:53Z
dc.date.available2022-08-23T16:54:53Z
dc.date.issued1999-02-26
dc.date.submitted2008-08-15
dc.identifier.citationJ Cell Biol. 1999 Feb 22;144(4):617-29. <a href="http://dx.doi.org/10.1083/jcb.144.4.617">Link to article on publisher's website</a>
dc.identifier.issn0021-9525 (Print)
dc.identifier.doi10.1083/jcb.144.4.617
dc.identifier.pmid10037785
dc.identifier.urihttp://hdl.handle.net/20.500.14038/42601
dc.description.abstractAnalysis of six endogenous pre-mRNAs demonstrates that localization at the periphery or within splicing factor-rich (SC-35) domains is not restricted to a few unusually abundant pre-mRNAs, but is apparently a more common paradigm of many protein-coding genes. Different genes are preferentially transcribed and their RNAs processed in different compartments relative to SC-35 domains. These differences do not simply correlate with the complexity, nuclear abundance, or position within overall nuclear space. The distribution of spliceosome assembly factor SC-35 did not simply mirror the distribution of individual pre-mRNAs, but rather suggested that individual domains contain both specific pre-mRNA(s) as well as excess splicing factors. This is consistent with a multifunctional compartment, to which some gene loci and their RNAs have access and others do not. Despite similar molar abundance in muscle fiber nuclei, nascent transcript "trees" of highly complex dystrophin RNA are cotranscriptionally spliced outside of SC-35 domains, whereas posttranscriptional "tracks" of more mature myosin heavy chain transcripts overlap domains. Further analyses supported that endogenous pre-mRNAs exhibit distinct structural organization that may reflect not only the expression and complexity of the gene, but also constraints of its chromosomal context and kinetics of its RNA metabolism.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=10037785&dopt=Abstract">Link to Article in PubMed</a>
dc.subjectCell Line
dc.subjectDystrophin
dc.subjectHumans
dc.subjectIn Situ Hybridization, Fluorescence
dc.subjectMyosin Heavy Chains
dc.subjectNuclear Proteins
dc.subjectRNA Precursors
dc.subjectRNA Processing, Post-Transcriptional
dc.subjectRNA Splicing
dc.subjectRNA, Messenger
dc.subject*Ribonucleoproteins
dc.subjectSpliceosomes
dc.subjectTranscription, Genetic
dc.subjectCell Biology
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleProcessing of endogenous pre-mRNAs in association with SC-35 domains is gene specific
dc.typeJournal Article
dc.source.journaltitleThe Journal of cell biology
dc.source.volume144
dc.source.issue4
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1933&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/934
dc.identifier.contextkey579824
refterms.dateFOA2022-08-23T16:54:53Z
html.description.abstract<p>Analysis of six endogenous pre-mRNAs demonstrates that localization at the periphery or within splicing factor-rich (SC-35) domains is not restricted to a few unusually abundant pre-mRNAs, but is apparently a more common paradigm of many protein-coding genes. Different genes are preferentially transcribed and their RNAs processed in different compartments relative to SC-35 domains. These differences do not simply correlate with the complexity, nuclear abundance, or position within overall nuclear space. The distribution of spliceosome assembly factor SC-35 did not simply mirror the distribution of individual pre-mRNAs, but rather suggested that individual domains contain both specific pre-mRNA(s) as well as excess splicing factors. This is consistent with a multifunctional compartment, to which some gene loci and their RNAs have access and others do not. Despite similar molar abundance in muscle fiber nuclei, nascent transcript "trees" of highly complex dystrophin RNA are cotranscriptionally spliced outside of SC-35 domains, whereas posttranscriptional "tracks" of more mature myosin heavy chain transcripts overlap domains. Further analyses supported that endogenous pre-mRNAs exhibit distinct structural organization that may reflect not only the expression and complexity of the gene, but also constraints of its chromosomal context and kinetics of its RNA metabolism.</p>
dc.identifier.submissionpathoapubs/934
dc.contributor.departmentDepartment of Cell Biology
dc.source.pages617-29


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