Functional analysis of Tpr: identification of nuclear pore complex association and nuclear localization domains and a role in mRNA export
Authors
Bangs, Peter LawrenceBurke, Brian
Powers, Christine M.
Craig, Roger W.
Purohit, Aruna
Doxsey, Stephen J.
Document Type
Journal ArticlePublication Date
1998-12-29Keywords
AnimalsBiological Transport
COS Cells
Cell Line
Cell Nucleus
Cricetinae
Hela Cells
Humans
Immunohistochemistry
Kidney
Macromolecular Substances
Mesocricetus
Microscopy, Fluorescence
Microscopy, Immunoelectron
Models, Biological
Nuclear Envelope
Nuclear Pore Complex Proteins
Nuclear Proteins
Protein Binding
*Protein Structure, Tertiary
Proteins
Proto-Oncogene Proteins
RNA, Messenger
Sequence Deletion
Structure-Activity Relationship
Transfection
Cell Biology
Metadata
Show full item recordAbstract
Tpr is a 270-kD coiled-coil protein localized to intranuclear filaments of the nuclear pore complex (NPC). The mechanism by which Tpr contributes to the structure and function of the nuclear pore is currently unknown. To gain insight into Tpr function, we expressed the full-length protein and several subdomains in mammalian cell lines and examined their effects on nuclear pore function. Through this analysis, we identified an NH2-terminal domain that was sufficient for association with the nucleoplasmic aspect of the NPC. In addition, we unexpectedly found that the acidic COOH terminus was efficiently transported into the nuclear interior, an event that was apparently mediated by a putative nuclear localization sequence. Ectopic expression of the full-length Tpr caused a dramatic accumulation of poly(A)+ RNA within the nucleus. Similar results were observed with domains that localized to the NPC and the nuclear interior. In contrast, expression of these proteins did not appear to affect nuclear import. These data are consistent with a model in which Tpr is tethered to intranuclear filaments of the NPC by its coiled coil domain leaving the acidic COOH terminus free to interact with soluble transport factors and mediate export of macromolecules from the nucleus.Source
J Cell Biol. 1998 Dec 28;143(7):1801-12.Permanent Link to this Item
http://hdl.handle.net/20.500.14038/42604PubMed ID
9864356Related Resources
Link to Article in PubMedRelated items
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