Interaction of ZPR1 with translation elongation factor-1alpha in proliferating cells
UMass Chan AffiliationsHoward Hughes Medical Institute and Program in Molecular Medicine
KeywordsAmino Acid Sequence
Molecular Sequence Data
Peptide Elongation Factor 1
Peptide Elongation Factors
*Saccharomyces cerevisiae Proteins
Sequence Homology, Amino Acid
MetadataShow full item record
AbstractThe zinc finger protein ZPR1 is present in the cytoplasm of quiescent mammalian cells and translocates to the nucleus upon treatment with mitogens, including epidermal growth factor (EGF). Homologues of ZPR1 were identified in yeast and mammals. These ZPR1 proteins bind to eukaryotic translation elongation factor-1alpha (eEF-1alpha). Studies of mammalian cells demonstrated that EGF treatment induces the interaction of ZPR1 with eEF-1alpha and the redistribution of both proteins to the nucleus. In the yeast Saccharomyces cerevisiae, genetic analysis demonstrated that ZPR1 is an essential gene. Deletion analysis demonstrated that the NH2-terminal region of ZPR1 is required for normal growth and that the COOH-terminal region was essential for viability in S. cerevisiae. The yeast ZPR1 protein redistributes from the cytoplasm to the nucleus in response to nutrient stimulation. Disruption of the binding of ZPR1 to eEF-1alpha by mutational analysis resulted in an accumulation of cells in the G2/M phase of cell cycle and defective growth. Reconstitution of the ZPR1 interaction with eEF-1alpha restored normal growth. We conclude that ZPR1 is essential for cell viability and that its interaction with eEF-1alpha contributes to normal cellular proliferation.
SourceJ Cell Biol. 1998 Dec 14;143(6):1471-84.
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/42605
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