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    Interaction of ZPR1 with translation elongation factor-1alpha in proliferating cells

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    Authors
    Gangwani, Laxman
    Mikrut, Monique
    Galcheva-Gargova, Zoya
    Davis, Roger J.
    UMass Chan Affiliations
    Howard Hughes Medical Institute and Program in Molecular Medicine
    Document Type
    Journal Article
    Publication Date
    1998-12-16
    Keywords
    Amino Acid Sequence
    Animals
    COS Cells
    Carrier Proteins
    Cell Cycle
    Cell Division
    Cell Line
    Consensus Sequence
    Fungal Proteins
    G2 Phase
    Gene Deletion
    Genes, Fungal
    Genotype
    Humans
    Mammals
    Mice
    Mitosis
    Molecular Sequence Data
    Peptide Elongation Factor 1
    Peptide Elongation Factors
    Recombinant Proteins
    Restriction Mapping
    Saccharomyces cerevisiae
    *Saccharomyces cerevisiae Proteins
    Schizosaccharomyces
    Sequence Alignment
    Sequence Homology, Amino Acid
    Zinc Fingers
    Cell Biology
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    Abstract
    The zinc finger protein ZPR1 is present in the cytoplasm of quiescent mammalian cells and translocates to the nucleus upon treatment with mitogens, including epidermal growth factor (EGF). Homologues of ZPR1 were identified in yeast and mammals. These ZPR1 proteins bind to eukaryotic translation elongation factor-1alpha (eEF-1alpha). Studies of mammalian cells demonstrated that EGF treatment induces the interaction of ZPR1 with eEF-1alpha and the redistribution of both proteins to the nucleus. In the yeast Saccharomyces cerevisiae, genetic analysis demonstrated that ZPR1 is an essential gene. Deletion analysis demonstrated that the NH2-terminal region of ZPR1 is required for normal growth and that the COOH-terminal region was essential for viability in S. cerevisiae. The yeast ZPR1 protein redistributes from the cytoplasm to the nucleus in response to nutrient stimulation. Disruption of the binding of ZPR1 to eEF-1alpha by mutational analysis resulted in an accumulation of cells in the G2/M phase of cell cycle and defective growth. Reconstitution of the ZPR1 interaction with eEF-1alpha restored normal growth. We conclude that ZPR1 is essential for cell viability and that its interaction with eEF-1alpha contributes to normal cellular proliferation.
    Source
    J Cell Biol. 1998 Dec 14;143(6):1471-84.
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/42605
    PubMed ID
    9852145
    Related Resources
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