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    Reversible endothelial cell relaxation induced by oxygen and glucose deprivation. A model of ischemia in vitro

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    Authors
    Doukas, John
    Cutler, Anne H.
    Boswell, Carl A.
    Joris, Isabelle
    Majno, G.
    UMass Chan Affiliations
    Department of Pathology
    Document Type
    Journal Article
    Publication Date
    1994-07-01
    Keywords
    Animals
    Cell Count
    Cell Hypoxia
    Cells, Cultured
    Endothelium, Vascular
    Glucose
    Ischemia
    Microfilament Proteins
    Models, Biological
    Muscle Contraction
    Muscle Relaxation
    Muscle, Smooth, Vascular
    Rats
    Time Factors
    Life Sciences
    Medicine and Health Sciences
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    Link to Full Text
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1887302/?tool=pubmed
    Abstract
    Endothelial cells (EC) cultured on polymerized silicone deform the underlying substrate, producing microscopically visible wrinkles. This has been interpreted as cellular contraction, and we have previously concluded that EC normally maintain an active contractile tone. Since in ischemic tissues capillaries become "paralyzed" and lose their tone, we decided to examine the effects of glucose and/or oxygen deprivation on EC contractility. Contracting cultures with wrinkled silicone substrates were exposed to complete anoxia with or without exogenous glucose and followed by time-lapse photography. Under either glucose-or oxygen-free conditions, contraction was maintained for up to 4 days. If, however, both oxygen and glucose were removed, cellular contraction was reversed. After a period of 2 to 4 hours substrate wrinkles gradually disappeared, until by 3 to 7 hours, few to no wrinkles remained. Furthermore, within 10 minutes of restoration to normal oxygen (but not glucose) levels, substrate wrinkling reappeared. F-actin microfilament patterns and cell number per unit area were also altered by glucose and oxygen deprivation. Similar results were obtained using large or small vessel EC. We conclude that in the absence of glucose and oxygen EC lose their contractile tone, and that tone can be re-established upon re-exposure to oxygen. These findings should have implications for the pathogenesis of capillary paralysis in ischemia.
    Source
    Am J Pathol. 1994 Jul;145(1):211-9.
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/42607
    PubMed ID
    8030750
    Related Resources
    Link to article in PubMed
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    UMass Chan Faculty and Researcher Publications

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