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dc.contributor.authorHinchcliffe, Edward H.
dc.contributor.authorCassels, Grizzel O.
dc.contributor.authorRieder, Conly L.
dc.contributor.authorSluder, Greenfield
dc.date2022-08-11T08:10:05.000
dc.date.accessioned2022-08-23T16:54:55Z
dc.date.available2022-08-23T16:54:55Z
dc.date.issued1998-04-29
dc.date.submitted2008-08-15
dc.identifier.citationJ Cell Biol. 1998 Mar 23;140(6):1417-26. <a href="http://dx.doi.org/10.1083/jcb.140.6.1417">Link to article on publisher's website</a>
dc.identifier.issn0021-9525 (Print)
dc.identifier.doi10.1083/jcb.140.6.1417
dc.identifier.pmid9508774
dc.identifier.urihttp://hdl.handle.net/20.500.14038/42609
dc.description.abstractCentrosomes repeatedly reproduce in sea urchin zygotes arrested in S phase, whether cyclin-dependent kinase 1-cyclin B (Cdk1-B) activity remains at prefertilization levels or rises to mitotic values. In contrast, when zygotes are arrested in mitosis using cyclin B Delta-90, anaphase occurs at the normal time, yet centrosomes do not reproduce. Together, these results reveal the cell cycle stage specificity for centrosome reproduction and demonstrate that neither the level nor the cycling of Cdk1-B activity coordinate centrosome reproduction with nuclear events. In addition, the proteolytic events of the metaphase-anaphase transition do not control when centrosomes duplicate. When we block protein synthesis at first prophase, the zygotes divide and arrest before second S phase. Both blastomeres contain just two complete centrosomes, which indicates that the cytoplasmic conditions between mitosis and S phase support centrosome reproduction. However, the fact that these daughter centrosomes do not reproduce again under such supportive conditions suggests that they are lacking a component required for reproduction. The repeated reproduction of centrosomes during S phase arrest points to the existence of a necessary "licensing" event that restores this component to daughter centrosomes during S phase, preparing them to reproduce in the next cell cycle.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=9508774&dopt=Abstract">Link to Article in PubMed</a>
dc.subjectAnaphase
dc.subjectAnimals
dc.subjectCDC28 Protein Kinase, S cerevisiae
dc.subjectCell Nucleus
dc.subjectCentrosome
dc.subjectFemale
dc.subjectMale
dc.subjectMetaphase
dc.subjectMicroscopy, Electron
dc.subjectMitosis
dc.subjectProphase
dc.subjectProtein Biosynthesis
dc.subjectProtein Kinases
dc.subjectProtein Synthesis Inhibitors
dc.subjectReproduction
dc.subjectS Phase
dc.subjectSea Urchins
dc.subjectZygote
dc.subjectCell Biology
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleThe coordination of centrosome reproduction with nuclear events of the cell cycle in the sea urchin zygote
dc.typeJournal Article
dc.source.journaltitleThe Journal of cell biology
dc.source.volume140
dc.source.issue6
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1940&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/941
dc.identifier.contextkey579831
refterms.dateFOA2022-08-23T16:54:56Z
html.description.abstract<p>Centrosomes repeatedly reproduce in sea urchin zygotes arrested in S phase, whether cyclin-dependent kinase 1-cyclin B (Cdk1-B) activity remains at prefertilization levels or rises to mitotic values. In contrast, when zygotes are arrested in mitosis using cyclin B Delta-90, anaphase occurs at the normal time, yet centrosomes do not reproduce. Together, these results reveal the cell cycle stage specificity for centrosome reproduction and demonstrate that neither the level nor the cycling of Cdk1-B activity coordinate centrosome reproduction with nuclear events. In addition, the proteolytic events of the metaphase-anaphase transition do not control when centrosomes duplicate. When we block protein synthesis at first prophase, the zygotes divide and arrest before second S phase. Both blastomeres contain just two complete centrosomes, which indicates that the cytoplasmic conditions between mitosis and S phase support centrosome reproduction. However, the fact that these daughter centrosomes do not reproduce again under such supportive conditions suggests that they are lacking a component required for reproduction. The repeated reproduction of centrosomes during S phase arrest points to the existence of a necessary "licensing" event that restores this component to daughter centrosomes during S phase, preparing them to reproduce in the next cell cycle.</p>
dc.identifier.submissionpathoapubs/941
dc.contributor.departmentDepartment of Cell Biology
dc.source.pages1417-26


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