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dc.contributor.authorJoyce, Terrence J.
dc.contributor.authorYood, Robert A.
dc.contributor.authorCarraway, Robert E.
dc.date2022-08-11T08:10:05.000
dc.date.accessioned2022-08-23T16:55:11Z
dc.date.available2022-08-23T16:55:11Z
dc.date.issued1993-09-01
dc.date.submitted2008-10-31
dc.identifier.citation<p>J Clin Endocrinol Metab. 1993 Sep;77(3):632-7.</p>
dc.identifier.issn0021-972X (Print)
dc.identifier.doi10.1210/jcem.77.3.7690359
dc.identifier.pmid7690359
dc.identifier.urihttp://hdl.handle.net/20.500.14038/42663
dc.description.abstractSubstance-P (SP) and its metabolites, SP-(1-7) and SP-(5-11), were quantitated in arthritic synovial fluids and plasma using a validated procedure. This process involved collection into appropriate enzyme inhibitors, extraction with acid-acetone, high pressure liquid chromatography, and RIA using region-specific antisera. Our results demonstrate that the levels of authentic SP in these fluids are less than 3.5 pmol/L, which is 50- to 10,000-fold less than those previously reported by others. These discrepant findings were not attributable to degradation, because added SP was recovered in good yield, and the measured levels of the metabolites SP-(1-7) and SP-(5-11) were also extremely low. In search of an explanation, we noted that many of the earlier reports involved direct assay of these fluids (without extraction and chromatography). Further work indicated that proteolytic enzymes (e.g. protease 24.11) present in these unextracted fluids can give rise to artifactually high SP measurements. We conclude that if SP is released within the joint space and if it participates in the inflammatory reaction and/or healing process, it most likely does so in a local fashion, which would not involve its accumulation in synovial fluid or plasma.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=7690359&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.1210/jcem.77.3.7690359
dc.subjectAdult
dc.subjectAged
dc.subjectAged, 80 and over
dc.subjectArthritis
dc.subjectChromatography, High Pressure Liquid
dc.subjectDrug Stability
dc.subjectEndopeptidases
dc.subjectFalse Positive Reactions
dc.subjectFemale
dc.subjectHumans
dc.subjectIndicators and Reagents
dc.subjectMale
dc.subjectMiddle Aged
dc.subjectPeptide Fragments
dc.subjectProtease Inhibitors
dc.subjectRadioimmunoassay
dc.subjectReference Values
dc.subjectSubstance P
dc.subjectSynovial Fluid
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleQuantitation of substance-P and its metabolites in plasma and synovial fluid from patients with arthritis
dc.typeJournal Article
dc.source.journaltitleThe Journal of clinical endocrinology and metabolism
dc.source.volume77
dc.source.issue3
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/990
dc.identifier.contextkey659173
html.description.abstract<p>Substance-P (SP) and its metabolites, SP-(1-7) and SP-(5-11), were quantitated in arthritic synovial fluids and plasma using a validated procedure. This process involved collection into appropriate enzyme inhibitors, extraction with acid-acetone, high pressure liquid chromatography, and RIA using region-specific antisera. Our results demonstrate that the levels of authentic SP in these fluids are less than 3.5 pmol/L, which is 50- to 10,000-fold less than those previously reported by others. These discrepant findings were not attributable to degradation, because added SP was recovered in good yield, and the measured levels of the metabolites SP-(1-7) and SP-(5-11) were also extremely low. In search of an explanation, we noted that many of the earlier reports involved direct assay of these fluids (without extraction and chromatography). Further work indicated that proteolytic enzymes (e.g. protease 24.11) present in these unextracted fluids can give rise to artifactually high SP measurements. We conclude that if SP is released within the joint space and if it participates in the inflammatory reaction and/or healing process, it most likely does so in a local fashion, which would not involve its accumulation in synovial fluid or plasma.</p>
dc.identifier.submissionpathoapubs/990
dc.contributor.departmentDepartment of Physiology
dc.source.pages632-7


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