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dc.contributor.authorYang, Kisuk
dc.contributor.authorGreiner, Dale L.
dc.contributor.authorKarp, Jeffrey M.
dc.date2022-08-11T08:10:06.000
dc.date.accessioned2022-08-23T16:55:32Z
dc.date.available2022-08-23T16:55:32Z
dc.date.issued2021-09-09
dc.date.submitted2022-06-14
dc.identifier.citation<p>Yang K, O'Cearbhaill ED, Liu SS, Zhou A, Chitnis GD, Hamilos AE, Xu J, Verma MKS, Giraldo JA, Kudo Y, Lee EA, Lee Y, Pop R, Langer R, Melton DA, Greiner DL, Karp JM. A therapeutic convection-enhanced macroencapsulation device for enhancing β cell viability and insulin secretion. Proc Natl Acad Sci U S A. 2021 Sep 14;118(37):e2101258118. doi: 10.1073/pnas.2101258118. PMID: 34504013; PMCID: PMC8449352. <a href="https://doi.org/10.1073/pnas.2101258118">Link to article on publisher's site</a></p>
dc.identifier.issn0027-8424 (Linking)
dc.identifier.doi10.1073/pnas.2101258118
dc.identifier.pmid34504013
dc.identifier.urihttp://hdl.handle.net/20.500.14038/42732
dc.description<p>Full author list omitted for brevity. For the full list of authors, see article.</p>
dc.description.abstractIslet transplantation for type 1 diabetes treatment has been limited by the need for lifelong immunosuppression regimens. This challenge has prompted the development of macroencapsulation devices (MEDs) to immunoprotect the transplanted islets. While promising, conventional MEDs are faced with insufficient transport of oxygen, glucose, and insulin because of the reliance on passive diffusion. Hence, these devices are constrained to two-dimensional, wafer-like geometries with limited loading capacity to maintain cells within a distance of passive diffusion. We hypothesized that convective nutrient transport could extend the loading capacity while also promoting cell viability, rapid glucose equilibration, and the physiological levels of insulin secretion. Here, we showed that convective transport improves nutrient delivery throughout the device and affords a three-dimensional capsule geometry that encapsulates 9.7-fold-more cells than conventional MEDs. Transplantation of a convection-enhanced MED (ceMED) containing insulin-secreting beta cells into immunocompetent, hyperglycemic rats demonstrated a rapid, vascular-independent, and glucose-stimulated insulin response, resulting in early amelioration of hyperglycemia, improved glucose tolerance, and reduced fibrosis. Finally, to address potential translational barriers, we outlined future steps necessary to optimize the ceMED design for long-term efficacy and clinical utility.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=34504013&dopt=Abstract">Link to Article in PubMed</a></p>
dc.rightsCopyright © 2021 the Author(s). Published by PNAS. This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectconvection
dc.subjectmacroencapsulation
dc.subjectstem cell–derived β cells
dc.subjecttype 1 diabetes
dc.subjectCell Biology
dc.subjectEndocrine System Diseases
dc.subjectEndocrinology
dc.subjectEndocrinology, Diabetes, and Metabolism
dc.subjectHormones, Hormone Substitutes, and Hormone Antagonists
dc.titleA therapeutic convection-enhanced macroencapsulation device for enhancing beta cell viability and insulin secretion
dc.typeJournal Article
dc.source.journaltitleProceedings of the National Academy of Sciences of the United States of America
dc.source.volume118
dc.source.issue37
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=6011&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/4976
dc.identifier.contextkey29715964
refterms.dateFOA2022-08-23T16:55:32Z
html.description.abstract<p>Islet transplantation for type 1 diabetes treatment has been limited by the need for lifelong immunosuppression regimens. This challenge has prompted the development of macroencapsulation devices (MEDs) to immunoprotect the transplanted islets. While promising, conventional MEDs are faced with insufficient transport of oxygen, glucose, and insulin because of the reliance on passive diffusion. Hence, these devices are constrained to two-dimensional, wafer-like geometries with limited loading capacity to maintain cells within a distance of passive diffusion. We hypothesized that convective nutrient transport could extend the loading capacity while also promoting cell viability, rapid glucose equilibration, and the physiological levels of insulin secretion. Here, we showed that convective transport improves nutrient delivery throughout the device and affords a three-dimensional capsule geometry that encapsulates 9.7-fold-more cells than conventional MEDs. Transplantation of a convection-enhanced MED (ceMED) containing insulin-secreting beta cells into immunocompetent, hyperglycemic rats demonstrated a rapid, vascular-independent, and glucose-stimulated insulin response, resulting in early amelioration of hyperglycemia, improved glucose tolerance, and reduced fibrosis. Finally, to address potential translational barriers, we outlined future steps necessary to optimize the ceMED design for long-term efficacy and clinical utility.</p>
dc.identifier.submissionpathoapubs/4976
dc.contributor.departmentProgram in Molecular Medicine, Diabetes Center of Excellence
dc.source.pagese2101258118


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Copyright © 2021 the Author(s). Published by PNAS. This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).
Except where otherwise noted, this item's license is described as Copyright © 2021 the Author(s). Published by PNAS. This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).