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Microinjection for precision genome editing in Caenorhabditis elegans

dc.contributor.authorGhanta, Krishna S.
dc.contributor.authorIshidate, Takao
dc.contributor.authorMello, Craig C.
dc.date2022-08-11T08:10:06.000
dc.date.accessioned2022-08-23T16:55:32Z
dc.date.available2022-08-23T16:55:32Z
dc.date.issued2021-09-01
dc.date.submitted2022-06-14
dc.identifier.citation<p>Ghanta KS, Ishidate T, Mello CC. Microinjection for precision genome editing in <em>Caenorhabditis elegans</em>. STAR Protoc. 2021 Sep 1;2(3):100748. doi: 10.1016/j.xpro.2021.100748. PMID: 34505086; PMCID: PMC8417391. <a href="https://doi.org/10.1016/j.xpro.2021.100748">Link to article on publisher's site</a></p>
dc.identifier.issn2666-1667 (Linking)
dc.identifier.doi10.1016/j.xpro.2021.100748
dc.identifier.pmid34505086
dc.identifier.urihttp://hdl.handle.net/20.500.14038/42734
dc.description.abstractIn Caenorhabditis elegans, targeted genome editing techniques are now routinely used to generate germline edits. The remarkable ease of C. elegans germline editing is attributed to the syncytial nature of the pachytene ovary which is easily accessed by microinjection. This protocol describes the step-by-step details and troubleshooting tips for the entire CRISPR-Cas genome editing procedure, including gRNA design and microinjection of ribonucleoprotein complexes, followed by screening and genotyping in C. elegans, to help accessing this powerful genetic animal system. For complete details on the use and execution of this protocol, please refer to Ghanta and Mello (2020).
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=34505086&dopt=Abstract">Link to Article in PubMed</a></p>
dc.rightsCopyright 2021. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCRISPR
dc.subjectgenetics
dc.subjectmodel organisms
dc.subjectmolecular biology
dc.subjectAmino Acids, Peptides, and Proteins
dc.subjectComputational Biology
dc.subjectGenomics
dc.subjectMolecular Biology
dc.subjectNucleic Acids, Nucleotides, and Nucleosides
dc.titleMicroinjection for precision genome editing in Caenorhabditis elegans
dc.typeJournal Article
dc.source.journaltitleSTAR protocols
dc.source.volume2
dc.source.issue3
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=6013&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/4978
dc.identifier.contextkey29715966
refterms.dateFOA2022-08-23T16:55:33Z
html.description.abstract<p>In Caenorhabditis elegans, targeted genome editing techniques are now routinely used to generate germline edits. The remarkable ease of C. elegans germline editing is attributed to the syncytial nature of the pachytene ovary which is easily accessed by microinjection. This protocol describes the step-by-step details and troubleshooting tips for the entire CRISPR-Cas genome editing procedure, including gRNA design and microinjection of ribonucleoprotein complexes, followed by screening and genotyping in C. elegans, to help accessing this powerful genetic animal system. For complete details on the use and execution of this protocol, please refer to Ghanta and Mello (2020).</p>
dc.identifier.submissionpathoapubs/4978
dc.contributor.departmentProgram in Molecular Medicine
dc.contributor.departmentRNA Therapeutics Institute
dc.source.pages100748


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Copyright 2021. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Except where otherwise noted, this item's license is described as Copyright 2021. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).