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Circulating Levels of sFlt1 Splice Variants as Predictive Markers for the Development of Preeclampsia

dc.contributor.authorSouders, Colby A.
dc.contributor.authorMaynard, Sharon E.
dc.contributor.authorYan, Jing
dc.contributor.authorWang, Yan
dc.contributor.authorBoatright, Naomi
dc.contributor.authorSedan, Jessica
dc.contributor.authorBalyozian, David
dc.contributor.authorCheslock, Peter S.
dc.contributor.authorMolrine, Deborah C.
dc.contributor.authorMoore Simas, Tiffany A.
dc.date2022-08-11T08:10:06.000
dc.date.accessioned2022-08-23T16:55:39Z
dc.date.available2022-08-23T16:55:39Z
dc.date.issued2015-06-02
dc.date.submitted2016-01-20
dc.identifier.citation<p>Int J Mol Sci. 2015 Jun 2;16(6):12436-53. doi: 10.3390/ijms160612436. <a href="http://dx.doi.org/10.3390/ijms160612436">Link to article on publisher's site</a></p>
dc.identifier.issn1422-0067 (Linking)
dc.identifier.doi10.3390/ijms160612436
dc.identifier.pmid26042465
dc.identifier.urihttp://hdl.handle.net/20.500.14038/42756
dc.description<p>This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</p>
dc.description.abstractAngiogenic biomarkers, including soluble fms-like tyrosine kinase 1 (sFlt1), are thought to be predictors of preeclampsia onset; however, improvement is needed before a widespread diagnostic test can be utilized. Here we describe the development and use of diagnostic monoclonal antibodies specific to the two main splice variants of sFlt1, sFlt1-1 and sFlt1-14. These antibodies were selected for their sensitivity and specificity to their respective sFlt1 isoform in a capture ELISA format. Data from this pilot study suggest that sFlt1-1 may be more predictive of preeclampsia than total sFlt1. It may be possible to improve current diagnostic platforms if more specific antibodies are utilized.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=26042465&dopt=Abstract">Link to Article in PubMed</a></p>
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectdiagnostic
dc.subjectisoforms
dc.subjectmonoclonal antibody (mAb)
dc.subjectpreeclampsia
dc.subjectsoluble fms-like tyrosine kinase 1 (sFlt1)
dc.subjectsplice variants
dc.subjectUMCCTS funding
dc.subjectFemale Urogenital Diseases and Pregnancy Complications
dc.subjectMaternal and Child Health
dc.subjectObstetrics and Gynecology
dc.subjectWomen's Health
dc.titleCirculating Levels of sFlt1 Splice Variants as Predictive Markers for the Development of Preeclampsia
dc.typeJournal Article
dc.source.journaltitleInternational journal of molecular sciences
dc.source.volume16
dc.source.issue6
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1107&amp;context=obgyn_pp&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/obgyn_pp/108
dc.identifier.contextkey8028977
refterms.dateFOA2022-08-23T16:55:40Z
html.description.abstract<p>Angiogenic biomarkers, including soluble fms-like tyrosine kinase 1 (sFlt1), are thought to be predictors of preeclampsia onset; however, improvement is needed before a widespread diagnostic test can be utilized. Here we describe the development and use of diagnostic monoclonal antibodies specific to the two main splice variants of sFlt1, sFlt1-1 and sFlt1-14. These antibodies were selected for their sensitivity and specificity to their respective sFlt1 isoform in a capture ELISA format. Data from this pilot study suggest that sFlt1-1 may be more predictive of preeclampsia than total sFlt1. It may be possible to improve current diagnostic platforms if more specific antibodies are utilized.</p>
dc.identifier.submissionpathobgyn_pp/108
dc.contributor.departmentDepartment of Biochemistry and Molecular Pharmacology
dc.contributor.departmentDepartment of Pediatrics, Division of Immunology and Infectious Disease
dc.contributor.departmentDepartment of Obstetrics and Gynecology
dc.contributor.departmentMassBiologics
dc.source.pages12436-53


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