Molecular cloning and characterization of rat CCL9 (MIP-1gamma), the ortholog of mouse CCL9
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UMass Chan Affiliations
Department of Cell BiologyDocument Type
Journal ArticlePublication Date
2005-05-28Keywords
Amino Acid SequenceAnimals
Base Sequence
Chemokines, CC
Chemotaxis
Cloning, Molecular
DNA, Complementary
Macrophage Inflammatory Proteins
Mice
Molecular Sequence Data
Osteoclasts
Polymerase Chain Reaction
RNA, Messenger
Rats
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
Species Specificity
Cell Biology
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We identified an EST sequence that was up-regulated during osteoclast formation in the rat. Investigating further, we cloned the cDNA from rat long bone and found it to be highly homologous to the mouse CC chemokine, CCL9, both at the nucleotide and amino acid levels. The rat CCL9 amino acid sequence is 74% identical to the mouse sequence, with an additional 11% similar amino acids. Recombinant rat CCL9 was used in chemotaxis assays of rat bone marrow cells and it was found to have a strong and dose-dependent effect. In addition, CCL9 mRNA was very highly up-regulated during osteoclast differentiation of rat bone marrow-derived mononuclear cells, increasing by over 100-fold when stimulated by colony stimulating factor-1 and the TNF superfamily member, RANKL. Together, these results establish that, like the mouse, the rat also uses CCL9 to promote the complex process of osteoclast formation.Source
Cytokine. 2005 Jul 21;31(2):94-102. Link to article on publisher's siteDOI
10.1016/j.cyto.2005.04.001Permanent Link to this Item
http://hdl.handle.net/20.500.14038/42872PubMed ID
15919212Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1016/j.cyto.2005.04.001
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