NHA-oc/NHA2: a mitochondrial cation-proton antiporter selectively expressed in osteoclasts
dc.contributor.author | Battaglino, Ricardo A. | |
dc.contributor.author | Pham, L. | |
dc.contributor.author | Morse, Leslie R. | |
dc.contributor.author | Vokes, M. | |
dc.contributor.author | Sharma, A. | |
dc.contributor.author | Odgren, Paul R. | |
dc.contributor.author | Yang, Meilheng | |
dc.contributor.author | Sasaki, Hajime | |
dc.contributor.author | Stashenko, Philip | |
dc.date | 2022-08-11T08:10:07.000 | |
dc.date.accessioned | 2022-08-23T16:56:12Z | |
dc.date.available | 2022-08-23T16:56:12Z | |
dc.date.issued | 2008-01-09 | |
dc.date.submitted | 2011-02-16 | |
dc.identifier.citation | Bone. 2008 Jan;42(1):180-92. Epub 2007 Sep 26. <a href="http://dx.doi.org/10.1016/j.bone.2007.09.046">Link to article on publisher's site</a> | |
dc.identifier.issn | 1873-2763 (Linking) | |
dc.identifier.doi | 10.1016/j.bone.2007.09.046 | |
dc.identifier.pmid | 17988971 | |
dc.identifier.uri | http://hdl.handle.net/20.500.14038/42877 | |
dc.description.abstract | Bone resorption is regulated by a complex system of hormones and cytokines that cause osteoblasts/stromal cells and lymphocytes to produce factors including RANKL, that ultimately result in the differentiation and activation of osteoclasts, the bone resorbing cells. We used a microarray approach to identify genes upregulated in RANKL-stimulated osteoclast precursor cells. Osteoclast expression was confirmed by multiple tissue Northern and in situ hybridization analysis. Gene function studies were carried out by siRNA analysis. We identified a novel gene, which we termed nha-oc/NHA2, which is strongly upregulated during RANKL-induced osteoclast differentiation in vitro and in vivo. nha-oc/NHA2 encodes a novel cation-proton antiporter (CPA) and is the mouse orthologue of a human gene identified in a database search: HsNHA2. nha-oc/NHA2 is selectively expressed in osteoclasts. NHA-oc/NHA2 protein localizes to the mitochondria, where it mediates Na(+)-dependent changes in mitochondrial pH and Na(+) acetate induced mitochondrial passive swelling. RNA silencing of nha-oc/nha2 reduces osteoclast differentiation and resorption, suggesting a role for NHA-oc/NHA2 in these processes. nha-oc/NHA2 therefore is a novel member of the CPA family and is the first mitochondrial NHA characterized to date. nha-oc/NHA2 is also unique in that it is the first eukaryotic and tissue-specific CPA2 characterized to date. NHA-oc/NHA2 displays the expected activities of a bona fide CPA and plays a key role(s) in normal osteoclast differentiation and function. | |
dc.language.iso | en_US | |
dc.relation | <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=17988971&dopt=Abstract">Link to Article in PubMed</a> | |
dc.relation.url | http://dx.doi.org/10.1016/j.bone.2007.09.046 | |
dc.subject | Amino Acid Sequence | |
dc.subject | Animals | |
dc.subject | Antiporters | |
dc.subject | Caspases | |
dc.subject | Cell Differentiation | |
dc.subject | Cell Line | |
dc.subject | Cloning, Molecular | |
dc.subject | Enzyme Activation | |
dc.subject | Gene Expression Regulation | |
dc.subject | Humans | |
dc.subject | Hydrogen-Ion Concentration | |
dc.subject | Mice | |
dc.subject | Mitochondria | |
dc.subject | Mitochondrial Swelling | |
dc.subject | Molecular Sequence Data | |
dc.subject | Osteoclasts | |
dc.subject | RNA, Messenger | |
dc.subject | RNA, Small Interfering | |
dc.subject | Cell Biology | |
dc.title | NHA-oc/NHA2: a mitochondrial cation-proton antiporter selectively expressed in osteoclasts | |
dc.type | Journal Article | |
dc.source.journaltitle | Bone | |
dc.source.volume | 42 | |
dc.source.issue | 1 | |
dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/odgren/6 | |
dc.identifier.contextkey | 1789901 | |
html.description.abstract | <p>Bone resorption is regulated by a complex system of hormones and cytokines that cause osteoblasts/stromal cells and lymphocytes to produce factors including RANKL, that ultimately result in the differentiation and activation of osteoclasts, the bone resorbing cells. We used a microarray approach to identify genes upregulated in RANKL-stimulated osteoclast precursor cells. Osteoclast expression was confirmed by multiple tissue Northern and in situ hybridization analysis. Gene function studies were carried out by siRNA analysis. We identified a novel gene, which we termed nha-oc/NHA2, which is strongly upregulated during RANKL-induced osteoclast differentiation in vitro and in vivo. nha-oc/NHA2 encodes a novel cation-proton antiporter (CPA) and is the mouse orthologue of a human gene identified in a database search: HsNHA2. nha-oc/NHA2 is selectively expressed in osteoclasts. NHA-oc/NHA2 protein localizes to the mitochondria, where it mediates Na(+)-dependent changes in mitochondrial pH and Na(+) acetate induced mitochondrial passive swelling. RNA silencing of nha-oc/nha2 reduces osteoclast differentiation and resorption, suggesting a role for NHA-oc/NHA2 in these processes. nha-oc/NHA2 therefore is a novel member of the CPA family and is the first mitochondrial NHA characterized to date. nha-oc/NHA2 is also unique in that it is the first eukaryotic and tissue-specific CPA2 characterized to date. NHA-oc/NHA2 displays the expected activities of a bona fide CPA and plays a key role(s) in normal osteoclast differentiation and function.</p> | |
dc.identifier.submissionpath | odgren/6 | |
dc.contributor.department | Department of Cell Biology | |
dc.source.pages | 180-92 |
Files in this item
This item appears in the following Collection(s)
-
Radiology Publications [1231]