Cytocompatible poly(ethylene glycol)-co-polycarbonate hydrogels cross-linked by copper-free, strain-promoted click chemistry
UMass Chan Affiliations
Department of Orthopedics and Physical RehabilitationDocument Type
Journal ArticlePublication Date
2011-10-01Keywords
AlkynesAnimals
Azides
Biocompatible Materials
Bone Marrow Cells
Cell Survival
*Click Chemistry
Cyclization
Heterocyclic Compounds, 3-Ring
Hydrogels
Male
Molecular Structure
Polyethylene Glycols
Rats
Biochemistry, Biophysics, and Structural Biology
Cell and Developmental Biology
Medicine and Health Sciences
Metadata
Show full item recordAbstract
Strategies to encapsulate cells in cytocompatible three-dimensional hydrogels with tunable mechanical properties and degradability without harmful gelling conditions are highly desired for regenerative medicine applications. Here we reported a method for preparing poly(ethylene glycol)-co-polycarbonate hydrogels through copper-free, strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry. Hydrogels with varying mechanical properties were formed by "clicking" azido-functionalized poly(ethylene glycol)-co-polycarbonate macromers with dibenzocyclooctyne-functionalized poly(ethylene glycol) under physiological conditions within minutes. Bone marrow stromal cells encapsulated in these gels exhibited higher cellular viability than those encapsulated in photo-cross-linked poly(ethylene glycol) dimethacrylate. The precise control over the macromer compositions, cytocompatible SPAAC cross-linking, and the degradability of the polycarbonate segments make these hydrogels promising candidates for scaffold and stem cell assisted tissue repair and regeneration.Source
Chem Asian J. 2011 Oct 4;6(10):2730-7. doi: 10.1002/asia.201100411. Epub 2011 Aug 24. Link to article on publisher's siteDOI
10.1002/asia.201100411Permanent Link to this Item
http://hdl.handle.net/20.500.14038/42969PubMed ID
21954076Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1002/asia.201100411