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    Dexamethasone modulates BMP-2 effects on mesenchymal stem cells in vitro

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    Authors
    Jager, Marcus
    Fischer, Johannes
    Dohrn, Wiebke
    Li, Xinning
    Ayers, David C.
    Czibere, Akos
    Prall, Wolf Christian
    Lensing-Hohn, Sabine
    Krauspe, Rudiger
    UMass Chan Affiliations
    Department of Orthopedics and Physical Rehabilitation
    Document Type
    Journal Article
    Publication Date
    2008-11-12
    Keywords
    Anti-Inflammatory Agents
    Biological Markers
    Bone Marrow Cells
    Bone Morphogenetic Protein 2
    Bone Morphogenetic Proteins
    Cell Differentiation
    Cell Proliferation
    Cell Survival
    Cells, Cultured
    Collagen Type I
    Dexamethasone
    Drug Combinations
    Fluorescent Antibody Technique, Indirect
    Gene Expression Regulation
    Humans
    Immunoenzyme Techniques
    Mesenchymal Stem Cells
    Osteoblasts
    Osteopontin
    RANK Ligand
    RNA, Messenger
    Transforming Growth Factor beta
    Orthopedics
    Rehabilitation and Therapy
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    Link to Full Text
    http://dx.doi.org/10.1002/jor.20565
    Abstract
    Dexamethasone/ascorbic acid/glycerolphosphate (DAG) and bone morphogenic protein (BMP)-2 are potent agents in cell proliferation and differentiation pathways. This study investigates the in vitro interactions between dexamethasone and BMP-2 for an osteoblastic differentiation of mesenchymal stem cells (MSCs). Bone marrow-derived human MSCs were cultured with DAG (group A), BMP-2 + DAG (group B), and DAG + BMP-2 combined with a porous collagen I/III scaffold (group C). RT-PCR, ELISA, immuncytochemical stainings and flow cytometry analysis served to evaluate the osteogenic-promoting potency of each of the above conditions in terms of cell morphology/viability, antigen presentation, and gene expression. DAG induced collagen I secretion from MSCs, which was further increased by the combination of DAG + BMP-2. In comparison, the collagen scaffold and the control samples showed no significant influence on collagen I secretion of MSCs. DAG stimulation of MSCs led also to a steady but not significant increase of BMP-2 level. A DAG and more, a DAG + BMP-2, stimulation increased the number of mesenchymal cells (CD105+/CD73+). All samples showed mRNA of ALP, osteopontin, Runx2, Twist 1 and 2, Notch-1/2, osteonectin, osteocalcin, BSP, and collagen-A1 after 28 days of in vitro culture. Culture media of all samples showed a decrease in Ca(2+) and PO(4) (2-) concentration, whereas a collagen-I-peak only occurred at day 28 in DAG- and DAG + BMP-2-stimulated bone marrow cells. In conclusion, BMP-2 enhances DAG-induced osteogenic differentiation in mesenchymal bone marrow cells. Both agents interact in various ways and can modify osteoblastic bone formation. Inc.
    Source
    J Orthop Res. 2008 Nov;26(11):1440-8. Link to article on publisher's site
    DOI
    10.1002/jor.20565
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/43027
    PubMed ID
    18404732
    Related Resources
    Link to Article in PubMed
    ae974a485f413a2113503eed53cd6c53
    10.1002/jor.20565
    Scopus Count
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    UMass Chan Faculty and Researcher Publications
    Orthopedics and Physical Rehabilitation Publications

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