Dexamethasone modulates BMP-2 effects on mesenchymal stem cells in vitro
Authors
Jager, MarcusFischer, Johannes
Dohrn, Wiebke
Li, Xinning
Ayers, David C.
Czibere, Akos
Prall, Wolf Christian
Lensing-Hohn, Sabine
Krauspe, Rudiger
UMass Chan Affiliations
Department of Orthopedics and Physical RehabilitationDocument Type
Journal ArticlePublication Date
2008-11-12Keywords
Anti-Inflammatory AgentsBiological Markers
Bone Marrow Cells
Bone Morphogenetic Protein 2
Bone Morphogenetic Proteins
Cell Differentiation
Cell Proliferation
Cell Survival
Cells, Cultured
Collagen Type I
Dexamethasone
Drug Combinations
Fluorescent Antibody Technique, Indirect
Gene Expression Regulation
Humans
Immunoenzyme Techniques
Mesenchymal Stem Cells
Osteoblasts
Osteopontin
RANK Ligand
RNA, Messenger
Transforming Growth Factor beta
Orthopedics
Rehabilitation and Therapy
Metadata
Show full item recordAbstract
Dexamethasone/ascorbic acid/glycerolphosphate (DAG) and bone morphogenic protein (BMP)-2 are potent agents in cell proliferation and differentiation pathways. This study investigates the in vitro interactions between dexamethasone and BMP-2 for an osteoblastic differentiation of mesenchymal stem cells (MSCs). Bone marrow-derived human MSCs were cultured with DAG (group A), BMP-2 + DAG (group B), and DAG + BMP-2 combined with a porous collagen I/III scaffold (group C). RT-PCR, ELISA, immuncytochemical stainings and flow cytometry analysis served to evaluate the osteogenic-promoting potency of each of the above conditions in terms of cell morphology/viability, antigen presentation, and gene expression. DAG induced collagen I secretion from MSCs, which was further increased by the combination of DAG + BMP-2. In comparison, the collagen scaffold and the control samples showed no significant influence on collagen I secretion of MSCs. DAG stimulation of MSCs led also to a steady but not significant increase of BMP-2 level. A DAG and more, a DAG + BMP-2, stimulation increased the number of mesenchymal cells (CD105+/CD73+). All samples showed mRNA of ALP, osteopontin, Runx2, Twist 1 and 2, Notch-1/2, osteonectin, osteocalcin, BSP, and collagen-A1 after 28 days of in vitro culture. Culture media of all samples showed a decrease in Ca(2+) and PO(4) (2-) concentration, whereas a collagen-I-peak only occurred at day 28 in DAG- and DAG + BMP-2-stimulated bone marrow cells. In conclusion, BMP-2 enhances DAG-induced osteogenic differentiation in mesenchymal bone marrow cells. Both agents interact in various ways and can modify osteoblastic bone formation. Inc.Source
J Orthop Res. 2008 Nov;26(11):1440-8. Link to article on publisher's siteDOI
10.1002/jor.20565Permanent Link to this Item
http://hdl.handle.net/20.500.14038/43027PubMed ID
18404732Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1002/jor.20565