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dc.contributor.authorJager, Marcus
dc.contributor.authorFischer, Johannes
dc.contributor.authorDohrn, Wiebke
dc.contributor.authorLi, Xinning
dc.contributor.authorAyers, David C.
dc.contributor.authorCzibere, Akos
dc.contributor.authorPrall, Wolf Christian
dc.contributor.authorLensing-Hohn, Sabine
dc.contributor.authorKrauspe, Rudiger
dc.date2022-08-11T08:10:08.000
dc.date.accessioned2022-08-23T16:56:53Z
dc.date.available2022-08-23T16:56:53Z
dc.date.issued2008-11-12
dc.date.submitted2011-05-26
dc.identifier.citationJ Orthop Res. 2008 Nov;26(11):1440-8. <a href="http://dx.doi.org/10.1002/jor.20565">Link to article on publisher's site</a>
dc.identifier.issn0736-0266 (Linking)
dc.identifier.doi10.1002/jor.20565
dc.identifier.pmid18404732
dc.identifier.urihttp://hdl.handle.net/20.500.14038/43027
dc.description.abstractDexamethasone/ascorbic acid/glycerolphosphate (DAG) and bone morphogenic protein (BMP)-2 are potent agents in cell proliferation and differentiation pathways. This study investigates the in vitro interactions between dexamethasone and BMP-2 for an osteoblastic differentiation of mesenchymal stem cells (MSCs). Bone marrow-derived human MSCs were cultured with DAG (group A), BMP-2 + DAG (group B), and DAG + BMP-2 combined with a porous collagen I/III scaffold (group C). RT-PCR, ELISA, immuncytochemical stainings and flow cytometry analysis served to evaluate the osteogenic-promoting potency of each of the above conditions in terms of cell morphology/viability, antigen presentation, and gene expression. DAG induced collagen I secretion from MSCs, which was further increased by the combination of DAG + BMP-2. In comparison, the collagen scaffold and the control samples showed no significant influence on collagen I secretion of MSCs. DAG stimulation of MSCs led also to a steady but not significant increase of BMP-2 level. A DAG and more, a DAG + BMP-2, stimulation increased the number of mesenchymal cells (CD105+/CD73+). All samples showed mRNA of ALP, osteopontin, Runx2, Twist 1 and 2, Notch-1/2, osteonectin, osteocalcin, BSP, and collagen-A1 after 28 days of in vitro culture. Culture media of all samples showed a decrease in Ca(2+) and PO(4) (2-) concentration, whereas a collagen-I-peak only occurred at day 28 in DAG- and DAG + BMP-2-stimulated bone marrow cells. In conclusion, BMP-2 enhances DAG-induced osteogenic differentiation in mesenchymal bone marrow cells. Both agents interact in various ways and can modify osteoblastic bone formation. Inc.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=18404732&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1002/jor.20565
dc.subjectAnti-Inflammatory Agents
dc.subjectBiological Markers
dc.subjectBone Marrow Cells
dc.subjectBone Morphogenetic Protein 2
dc.subjectBone Morphogenetic Proteins
dc.subjectCell Differentiation
dc.subjectCell Proliferation
dc.subjectCell Survival
dc.subjectCells, Cultured
dc.subjectCollagen Type I
dc.subjectDexamethasone
dc.subjectDrug Combinations
dc.subjectFluorescent Antibody Technique, Indirect
dc.subjectGene Expression Regulation
dc.subjectHumans
dc.subjectImmunoenzyme Techniques
dc.subjectMesenchymal Stem Cells
dc.subjectOsteoblasts
dc.subjectOsteopontin
dc.subjectRANK Ligand
dc.subjectRNA, Messenger
dc.subjectTransforming Growth Factor beta
dc.subjectOrthopedics
dc.subjectRehabilitation and Therapy
dc.titleDexamethasone modulates BMP-2 effects on mesenchymal stem cells in vitro
dc.typeJournal Article
dc.source.journaltitleJournal of orthopaedic research : official publication of the Orthopaedic Research Society
dc.source.volume26
dc.source.issue11
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/ortho_pp/35
dc.identifier.contextkey2032274
html.description.abstract<p>Dexamethasone/ascorbic acid/glycerolphosphate (DAG) and bone morphogenic protein (BMP)-2 are potent agents in cell proliferation and differentiation pathways. This study investigates the in vitro interactions between dexamethasone and BMP-2 for an osteoblastic differentiation of mesenchymal stem cells (MSCs). Bone marrow-derived human MSCs were cultured with DAG (group A), BMP-2 + DAG (group B), and DAG + BMP-2 combined with a porous collagen I/III scaffold (group C). RT-PCR, ELISA, immuncytochemical stainings and flow cytometry analysis served to evaluate the osteogenic-promoting potency of each of the above conditions in terms of cell morphology/viability, antigen presentation, and gene expression. DAG induced collagen I secretion from MSCs, which was further increased by the combination of DAG + BMP-2. In comparison, the collagen scaffold and the control samples showed no significant influence on collagen I secretion of MSCs. DAG stimulation of MSCs led also to a steady but not significant increase of BMP-2 level. A DAG and more, a DAG + BMP-2, stimulation increased the number of mesenchymal cells (CD105+/CD73+). All samples showed mRNA of ALP, osteopontin, Runx2, Twist 1 and 2, Notch-1/2, osteonectin, osteocalcin, BSP, and collagen-A1 after 28 days of in vitro culture. Culture media of all samples showed a decrease in Ca(2+) and PO(4) (2-) concentration, whereas a collagen-I-peak only occurred at day 28 in DAG- and DAG + BMP-2-stimulated bone marrow cells. In conclusion, BMP-2 enhances DAG-induced osteogenic differentiation in mesenchymal bone marrow cells. Both agents interact in various ways and can modify osteoblastic bone formation. Inc.</p>
dc.identifier.submissionpathortho_pp/35
dc.contributor.departmentDepartment of Orthopedics and Physical Rehabilitation
dc.source.pages1440-8


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