• Login
    View Item 
    •   Home
    • UMass Chan Departments, Programs and Centers
    • Orthopedics and Physical Rehabilitation Publications
    • View Item
    •   Home
    • UMass Chan Departments, Programs and Centers
    • Orthopedics and Physical Rehabilitation Publications
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of eScholarship@UMassChanCommunitiesPublication DateAuthorsUMass Chan AffiliationsTitlesDocument TypesKeywordsThis CollectionPublication DateAuthorsUMass Chan AffiliationsTitlesDocument TypesKeywords

    My Account

    LoginRegister

    Help

    AboutSubmission GuidelinesData Deposit PolicySearchingAccessibilityTerms of UseWebsite Migration FAQ

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Functional glass slides for in vitro evaluation of interactions between bone cells and mineral-binding surfaces

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Journal_Materials_Chem_2004_So ...
    Size:
    593.9Kb
    Format:
    PDF
    Download
    Authors
    Song, Jie
    Chen, Julia
    Klapperich, Catherine M.
    Eng, Vincent
    Bertozzi, Carolyn R.
    UMass Chan Affiliations
    Department of Cell Biology
    Department of Orthopedics and Physical Rehabilitation
    Document Type
    Journal Article
    Publication Date
    2004-08-01
    Keywords
    Biocompatible Materials
    Bone Substitutes
    Tissue Scaffolds
    Orthopedics
    Rehabilitation and Therapy
    
    Metadata
    Show full item record
    Abstract
    Primary amine-functionalized glass slides obtained through a multi-step plasma treatment were conjugated with anionic amino acids that are frequently found as mineral binding elements in acidic extracellular matrix components of natural bone. The modified glass surfaces were characterized by X-ray photoelectron spectroscopy (XPS) and contact angle measurements. Human osteosarcoma TE85 cells were cultured on these functionalized slides and analyses on both protein and gene expression levels were performed to probe the ‘‘biocompatibility’’ of the surface ligands. Cell attachment and proliferation on anionic surfaces were either better than or comparable to those of cells cultured on tissue culture polystyrene (TCPS). The modified glass surfaces promoted the expression of osteocalcin, alkaline phosphatase activity and ECM proteins such as fibronectin and vitronectin under differentiation culture conditions. Transcript analysis using gene chip microarrays confirmed that culturing TE85 cells on anionic surfaces did not activate apoptotic pathways. Collectively, these results suggest that the potential mineral-binding anionic ligands examined here do not exert significant adverse effects on the expression of important osteogenic markers of TE85 cells. This work paves the way for the incorporation of these ligands into 3-dimensional artificial bone-like scaffolds.
    Source
    Song J, Chen J, Klapperich CM, Eng V, Bertozzi CR. Functional glass slides for in vitro evaluation of interactions between bone cells and mineral-binding surfaces. J Mater Chem. 2004 Jan; 14: 2643-2648. Link to article on publisher's website
    DOI
    10.1039/B408597N
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/43072
    Notes

    At the time of publication, Jie Song was not yet affiliated with the University of Massachusetts Medical School.

    ae974a485f413a2113503eed53cd6c53
    10.1039/B408597N
    Scopus Count
    Collections
    UMass Chan Faculty and Researcher Publications
    Orthopedics and Physical Rehabilitation Publications

    entitlement

     
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Lamar Soutter Library, UMass Chan Medical School | 55 Lake Avenue North | Worcester, MA 01655 USA
    Quick Guide | escholarship@umassmed.edu
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.