Effect of perineal cleansing on contamination rate of mid-stream urine culture
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UMass Chan Affiliations
Department of PediatricsDocument Type
Journal ArticlePublication Date
2006-02-14Keywords
AdolescentAdult
Antisepsis
Bacteriuria
Colony Count, Microbial
Female
Humans
Perineum
Specimen Handling
Urinary Tract Infections
Urine
Pediatrics
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STUDY OBJECTIVE: Urinary tract infection (UTI) and chlamydial or gonococcal urethritis are the most common causes of female dysuria. While chlamydia and gonorrhea can be detected with a nucleic acid amplification test performed on an uncleansed first part voided urine sample, urine cultures to test for UTI require a mid-stream clean caught sample. In order to determine whether collecting two sequential non-clean caught urine samples during the same void to test for chlamydia, gonorrhea, and UTI is a reasonable approach, we assessed the degree to which perineal cleansing reduces bacterial contamination of mid-stream urine cultures. DESIGN: Experimental study comparing mid-stream urine samples collected with (n = 25) and without (n = 25) perineal cleansing. SETTING: A university-based adolescent clinic PARTICIPANTS: We recruited fifty 14-23-year-old (mean 18.5 yrs, SD 2.3) asymptomatic females. MAIN OUTCOME MEASURE: Perineal flora contamination rate of mid-stream urine cultures collected with and without perineal cleansing. RESULTS: No culture grew >10(4) colonies of a pathogenic bacterium. Eleven (44%) of the experimental group samples and 9 (36%) of the control samples grew >10(4) colonies of perineal bacterial flora (chi(2) = .33, P = 0.56). Participants' previous experience collecting mid-stream urine was not associated with less bacterial contamination. CONCLUSIONS: Among this small sample of asymptomatic young women, perineal cleansing did not significantly reduce perineal flora contamination of mid-stream urine cultures. If larger studies of symptomatic young women replicate these findings, young women could collect two sequential urine samples from the same void to test for chlamydia, gonorrhea, and UTI.Source
J Pediatr Adolesc Gynecol. 2006 Feb;19(1):31-4. Link to article on publisher's siteDOI
10.1016/j.jpag.2005.11.003Permanent Link to this Item
http://hdl.handle.net/20.500.14038/43110PubMed ID
16472726Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1016/j.jpag.2005.11.003