Murine model for cystic fibrosis bone disease demonstrates osteopenia and sex-related differences in bone formation
AuthorsPashuck, Troy D.
Franz, Sarah E.
Altman, Molly K.
Wasserfall, Clive H.
Atkinson, Mark A.
Wronski, Thomas J.
Flotte, Terence R.
Stalvey, Michael S.
UMass Chan AffiliationsDepartment of Pediatrics
Body Weights and Measures
Bone Diseases, Metabolic
Bone and Bones
Cystic Fibrosis Transmembrane Conductance Regulator
*Disease Models, Animal
Mice, Inbred C57BL
Endocrinology, Diabetes, and Metabolism
MetadataShow full item record
AbstractAs the incidence of cystic fibrosis (CF) bone disease is increasing, we analyzed CF transmembrane conductance regulator (CFTR) deficient mice (CF mice) to gain pathogenic insights. In these studies comparing adult (14 wk) CF and C57BL/6J mice, both bone length and total area were decreased in CF mice. Metaphyseal trabecular and cortical density were also decreased, as well as diaphyseal cortical and total density. Trabecular bone volume was diminished in CF mice. Female CF mice revealed decreased trabecular width and number compared with C57BL/6J, whereas males demonstrated no difference in trabecular number. Female CF mice had reduced mineralizing surface and bone formation rates. Conversely, male CF mice had increased mineralizing surface, mineral apposition, and bone formation rates compared with C57BL/6J males. Bone formation rate was greater in males compared with female CF mice. Smaller bones with decreased density in CF, despite absent differences in osteoblast and osteoclast surfaces, suggest CF transmembrane conductance regulator influences bone cell activity rather than number. Differences in bone formation rate in CF mice are suggestive of inadequate bone formation in females but increased bone formation in males. This proanabolic observation in male CF mice is consistent with other clinical sex differences in CF.
SourcePediatr Res. 2009 Mar;65(3):311-6. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/43195
Related ResourcesLink to Article in PubMed